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[Objective] To study the content of polysaccharides in 3 Huoshan species of Dendrobiumn with the variation of time.[Method] The content of polysaccharides in 3 species of Dendrobiumn including D.officinale,D.huoshanense and D.moniliforme at 8 growth stages was determined by sulfuric acid-phenol method.And,the changing trend and impacting factors were also analyzed.[Result] The content of polysaccharides was increasing steadily based on a low level at the stage of tissue culture,and it decreased in the following order,D.officinale,D.huoshanense,D.moniliforme.The highest content of polysaccharides in those Dendrobium was pursued after two years culture,the order was D.huoshanense > D.officinale >D.moniliforme,among which the content of polysaccharides in D.huoshanense was as high as 35.74%.[Conclusion] Those results lay a foundation for the breeding,cultivation and application of those three kinds of Dendrobium.
[Objective] To study the content of polysaccharides in 3 Huoshan species of Dendrobium n with the variation of time. [Method] The content of polysaccharides in 3 species of Dendrobium n including D. officinale, D. huoshanense and D. moniliforme at 8 growth stages was determined by sulfuric acid-phenol method. And, the changing trend and impacting factors were also analyzed. [Result] The content of polysaccharides was increased steadily based on a low level at the stage of tissue culture, and it decreased in the following order, D. officicinale, D. huoshanense, D. moniliforme. Highest content of polysaccharides in those Dendrobium was pursued after two years culture, the order was D. huoshanense> D. officinale> D. moniliforme, among which the content of polysaccharides in D .huoshanense was as high as 35.74%. Conclusion The results lay a foundation for the breeding, cultivation and application of those three kinds of Dendrobium.