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Aim:The aim of this study was to investigate the effects of aloe-emodin,a naturalcompound from the root and rhizome of Rheum palmatum,on the growth of hu-man cervical cancer cells,HeLa.Methods:HeLa ceils were treated with variousconcentrations of aloe-emodin for 1-5 d,and cell growth was measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay.The long-termgrowth effect was investigated by crystal violet assay.The distributions of thecell cycle and apoptosis were analyzed by flow cytometry.The alkaline phos-phatase (ALP) activity was analyzed by a chemical analyzer.Finally,Westernblotting was used to indicate the abundant changes of protein kinase C (PKC),c-myc,cyclins,cyclin-dependent kinases (CDK),and proliferating cell nuclear anti-gen (PCNA).Results:Aloe-emodin inhibited the growth of HeLa cells in a dose-dependent manner at concentrations ranging between 2.5 and 40 μmol/L.Theflow cytometric analysis showed that HeLa cells were arrested at the G_2/M phase.This effect was associated with the decrease in cyclin A and CDK2,and theincrease in cyclin B1 and CDK1.More importantly,the ALP activity was found tobe increased by aloe-emodin treatment,and accompanied by the inhibition ofPCNA expression.In addition,aloe-emodin suppressed the expression of PKCαand c-myc.Conclusion:These findings provide a possible mechanistic explana-tion for the growth inhibitory effect of aloe-emodin on HeLa,which includes cellcycle arrest and inducing differentiation.
Aim: The aim of this study was to investigate the effects of aloe-emodin, a naturalcompound from the root and rhizome of Rheum palmatum, on the growth of hu-man cervical cancer cells, HeLa. Methods: HeLa ceils were treated with variousconcentrations of aloe-emodin for 1-5 d, and cell growth was measured by 3- (4,5-dimethylthiazol-2-yl) -2,5-diphenyl tetrazolium bromide assay.The long- term growth effect was investigated by crystal violet assay. The distributions of the cell cycle and apoptosis were analyzed by flow cytometry. The alkaline phos-phatase (ALP) activity was analyzed by a chemical analyzer. Finaally, Western blotting was used to indicate the abundant changes of protein kinase C (PKC), c- myc , cyclins, cyclin-dependent kinases (CDKs), and proliferating cell nuclear anti-gen (PCNA). Results: Aloe-emodin inhibited the growth of HeLa cells in a dose-dependent manner at concentrations ranging between 2.5 and 40 μmol / L. Theflow cytometric analysis showed that HeLa cells were arrested at the G_2 / M phase.Th is effect was associated with the decrease in cyclin A and CDK2, and the increase in cyclin B1 and CDK1.More importantly, the ALP activity was found to be increased by aloe-emodin treatment, and accompanied by the inhibition ofPCNA expression. addition, aloe- emodin suppressed the expression of PKCαand c-myc.Conclusion: These findings provide a probable mechanistic explana tion for the growth inhibitory effect of aloe-emodin on HeLa, which includes cellcycle arrest and inducing differentiation.