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以甘肃贝母地下鳞茎为试材,研究了不同消毒方法、不同激素组合对其鳞茎的诱导增殖及植株再生的影响,以期建立甘肃贝母的快速无性繁殖体系。结果表明:用70%乙醇处理15s,0.1%升汞处理10min,无菌水洗5次,然后在4℃中冷藏过夜,第2天重复上述灭菌步骤的连续消毒的方式灭菌效果很好,其染菌率为16.7%;诱导鳞茎不定芽的最佳培养基是MS+NAA2.0mg/L+6-BA 0.5mg/L,出芽率较高,为87.5%,能分化出的绿色健壮的不定芽;继代增殖培养基是MS+NAA 0.5mg/L+6-BA 2.0mg/L,增殖系数达到9;生根的最佳培养基是1/2MS+NAA 0.5mg/L,生根率90%。该试验基本建立了甘肃贝母植株的再生体系,为其愈伤组织的增殖和完整植株的再生提供了重要的研究基础。
The bulb of Gansu Fritillaria was used as test material to study the effects of different sterilizing methods and combinations of different hormones on its bulblet proliferation and plant regeneration in order to establish a rapid clonal reproductive system of Fritillaria gansuensis. The results showed that sterilization with 70% ethanol for 15s, 0.1% mercuric chloride for 10min, sterile water washing for 5 times and then overnight at 4 ℃, The rate of infection was 16.7%. The best medium for inducing adventitious buds was MS + NAA 2.0 mg / L + 6-BA 0.5 mg / L, with a high germination rate of 87.5% Adventitious buds. The proliferation medium was MS + NAA 0.5mg / L + 6-BA 2.0mg / L, the multiplication coefficient reached 9; the best rooting medium was 1/2 MS + NAA 0.5mg / L, %. The experiment basically established the regeneration system of Fritillaria cirrhosa plants in Gansu Province and provided an important research foundation for the proliferation of callus and the regeneration of intact plants.