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[Objective] The study aimed to establish an efficient method to extract RNA from cassava,and clone the core sequence of SSS II gene. [Method] The cassava RNA was obtained using the modified CTAB method,which was then reversely transcripted into cDNA. Degenerate primers were designed based on the homology property of known SSS II sequences in other plant species. A fragment was amplified with the previously mentioned cDNA as template and the degenerate primers through Polymerase Chain Reaction ( PCR) . [Result] After online blasting in NCBI,the sequence was identified as the core fragment of cassava SSS II gene. [Conclusion] Our research would lay the original basis for the cloning of the cassava SSS II full length cDNA sequence and construction of its antisense vector,which could further provide proper candidate genes for the development of starch metabolic engineering.
[Objective] The study aimed to establish an efficient method to extract RNA from cassava, and clone the core sequence of SSS II gene. [Method] The cassava RNA was obtained using the modified CTAB method, which was then reversely transcripted into cDNA. Degenerate primers were designed based on the homology property of the known SSS II sequences in other plant species. A fragment was amplified with the previously mentioned cDNA as template and the degenerate primers through Polymerase Chain Reaction (PCR). [Result] After online blasting in NCBI, the sequence was identified as the core fragment of cassava SSS II gene. [Conclusion] Our research would lay the original basis for the cloning of the cassava SSS II full length cDNA sequence and construction of its antisense vector, which could further provide proper candidate genes for the development of starch metabolic engineering.