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目的探讨大肠癌血管新生、嵌合体血管(MV)与临床分期之间的关系及临床意义。方法对1999～2002北京朝阳医院消化科49例大肠癌患者的病变组织切片,用抗CD31单克隆抗体标记血管内皮细胞;用抗CEA单克隆抗体标记结肠癌细胞。经免疫组织化学双标染色检测患者瘤组织“热点”区域的微血管密度(MVD)、MV,并分析其与Dukes临床各期的关系。结果在不同Dukes分期的大肠癌组织标本中,MVD计数分别为B期43.98±21.46,C期59.54±26.95,D期70.80±19.04,表明大肠癌组织MVD随疾病分期的升高而增加,D期明显高于B期(P<0.01)。MV在B期占40.0%;C期占60.0%;D期占87.5%,表明MV随大肠癌的临床分期的增加而增加。经随访发现,随着大肠癌分期、MVD及MV的增加,患者生存率降低,生存期短。表明MVD与大肠癌的预后相关。结论大肠癌MVD及MV与疾病的分期及预后有关,提示临床对高MVD及MV患者应积极治疗。大肠癌血管新生及MV的存在为临床治疗提供了新的靶位点。
Objective To investigate the relationship and clinical significance of angiogenesis, chimerism and colon cancer in colorectal cancer. Methods The lesions of 49 patients with colorectal cancer in Department of Gastroenterology of Beijing Chaoyang Hospital from 1999 to 2002 were stained with anti-CD31 monoclonal antibody and colon cancer cells were labeled with anti-CEA monoclonal antibody. The microvessel density (MVD) and MV in the “hot spot” region of tumor tissue were detected by immunohistochemical double-labeled staining, and the relationship between them and the clinical stages of Dukes was analyzed. Results In colorectal cancer samples with different Dukes stages, the MVD counts were 43.98 ± 21.46 in stage B, 59.54 ± 26.95 in stage C and 70.80 ± 19.04 in stage D, respectively, indicating that the MVD in colorectal cancer tissues increased with the stage of disease, Obviously higher than B (P <0.01). MV was 40.0% in stage B, 60.0% in stage C, and 87.5% in stage D, indicating that MV increased with the clinical stage of colorectal cancer. Follow-up found that, with the staging of colorectal cancer, MVD and MV increased, the survival rate of patients with reduced, short survival. MVD and prognosis of colorectal cancer. Conclusions MVD and MV in colorectal cancer are related to the stage and prognosis of the disease, suggesting that clinical treatment of patients with high MVD and MV should be actively treated. The presence of angiogenesis and MV in colorectal cancer provides a new target site for clinical treatment.