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本文观察37例小儿单纯性肾病(INS)、6例急性链球菌感染后肾炎(APSGN)血浆及6例INS外周血单个核细胞(PBMC)培养上清液对SAC诱导的正常人B细胞增殖的影响,8例INS血浆对PWM诱导B细胞产生IgG及其亚类的影响。结果发现INS发作期血浆抑制B细胞增殖及PWM诱导的B细胞分化,对B细胞增殖的抑制效应不能被正常人混合血浆及小牛血清纠正,表明INS血浆抑制作用是由于确有抑制因子存在,而非缺乏某种物质。血浆抑制作用在INS缓解期消失,正常儿童及APSGN儿童血浆无此抑制作用。INS发作期血浆与其PBMC上清液对B细胞增殖的抑制作用呈直线正相关,提示其血浆抑制因子可能为INS患儿PBMC的产物。
In this study, we observed the effects of 37 cases of children with simple nephropathy (INS), 6 cases of acute streptococcal nephritis (APSGN) plasma and 6 cases of INS peripheral blood mononuclear cells (PBMC) supernatant on SAC-induced normal human B cell proliferation Influence of 8 cases of INS plasma on the production of IgG and its subclasses by PWM-induced B cells. The results found that INS attack plasma B cells inhibit proliferation and PWM-induced B cell differentiation, the inhibitory effect of B cell proliferation can not be normal mixed plasma and calf serum corrected, indicating that INS plasma inhibition is due to the presence of inhibitors, Not lack of something. Plasma inhibition disappeared in the remission of INS, normal children and APSGN plasma no such inhibition. There was a linear positive correlation between the inhibitory effect of plasma and PBMC supernatant on the proliferation of B cells during INS attack, suggesting that the plasma inhibitor may be the product of PBMC in children with INS.