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本文采用正交设计L_(25)~((5)4)对影响大鼠腹腔巨噬细胞(PMφ)鲁米诺依赖的化学发光(LDCL)实验因素—鲁米诺浓度、细胞浓度、酵母多糖浓度、酵母多糖调理时间等进行了探讨,表明其最佳反应组合水平为:100μmol/L—5×10~6/ml—10g/L—45min大鼠腹腔Mφ在有或无白芍总甙(TGP)的条件下体外培养12h,用上述各因素的亚适水平进行LDCL测定,结果表明,TGP在0.09—2.25mg/L范围内可浓度依赖性地增加Mφ的LDCL强度,当TGP浓度增至11.25mg/L时,CL降低,量效曲线呈钟罩形趋势,提示该药对LDCL的影响有双向作用。
In this paper, orthogonal design L_(25)~((5)4) was used to investigate the luminol-dependent chemiluminescence (LDCL) factors affecting rat peritoneal macrophages (PMφ)—luminol concentration, cell concentration, and zymosan The concentrations and the conditioning time of zymosan were investigated. The results showed that the optimal reaction combination level was 100μmol/L-5x10-6/ml-10g/L-45min rat abdominal cavity Mφ with or without total tinea falciparum ( Under the condition of TGP) cultured in vitro for 12h, the LDCL was measured by the sub-appropriate level of the above factors. The results showed that TGP could increase the LDCL intensity of Mφ in a concentration-dependent manner within the range of 0.09-2.25mg/L, when the concentration of TGP increased to At 11.25 mg/L, CL decreased, and the dose-response curve showed a bell-shaped trend, suggesting that the drug had a bidirectional effect on LDCL.