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1987年我们从广安县一例肾综合征出血热(HFRS)患者腹膜渗出细胞分离出汉坦病毒(HV)GH716。这株病毒为HV属的Ⅰ型病毒,即抗原型别属野鼠型。以后,我们引进了国际Ⅰ型代表毒株76-118和Ⅱ型毒株R22。1987-1994年的8年中,我们用这3株病毒感染非洲绿猴肾细胞(VeroE6),制备了188张HV抗原(HVAg)片。另外,从疫区HV+鼠制备了HVAg+鼠肺抗原片222张。上述两种抗原片共计410张,4500孔。这些抗原片用于HFRS研究、临床患者确诊、疑似病例的鉴别诊断和人群HV感染的血清流行病学监测,提高了HFRS的科学研究和防治水平。
In 1987, we isolated Hantaan virus (HV) GH716 from peritoneal exudate cells from a patient with renal syndrome hemorrhagic fever (HFRS) in Guang’an County. This strain of virus is a type I virus of the genus HV, that is, the genotype of the antigen is wild-type. Later, we introduced the international type I strain 76-118 and the type II strain R22.1987-1994-1994 years, we use these three strains of virus infection in African green monkey kidney cells (VeroE6), prepared 188 HV antigen (HVAg) tablets. In addition, 222 HV HV + mouse lung antigens were prepared from the infected HV + mice. A total of 410 tablets of the two antigens, 4500 holes. These antigens have been used in HFRS research, diagnosis of clinical patients, differential diagnosis of suspected cases and serological epidemiological surveillance of human HV infection, raising the scientific research and prevention and treatment of HFRS.