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目的:建立同时测定板蓝根颗粒中4种核苷类成分含量的快速液相色谱方法。方法:色谱柱:Agilent Zorbox SBC18(4.6×50mm,1.8μm),流动相:水(A)-甲醇(B)线性梯度洗脱[0-4min,0-3%B;4-12min,3-12%B;12-16min,50-50%B;16-20min,0-0%B],流速:0.6mL.min-1,柱温:30℃,检测波长:254nm。结果:尿苷的线性范围为2.164～86.56μg,r=0.9997;鸟苷的线性范围为2.49～99.6μg,r=0.9999;表告依春的线性范围为3.696～147.84μg,r=0.9999;腺苷的线性范围为2.476～99.040μg,r=0.9999。尿苷、鸟苷、表告伊春及腺苷平均回收率分别为为95.9%,99.9%,101.0%,99.8%,平均RSD分别为0.8%,1.8%,1.3%,0.8%(n=6)。5家企业的的板蓝根颗粒中核苷类成分的含量存在差异。结论:该方法操作简单,重复性好,灵敏度高,结果准确可靠。
Objective: To establish a rapid liquid chromatographic method for simultaneous determination of 4 nucleosides in Banlangen granules. Methods: The column was Agilent Zorbox SBC18 (4.6 × 50 mm, 1.8 μm). The mobile phase consisted of a linear gradient of water (A) -methanol (B) [0-4min, 0-3% 12% B; 12-16min, 50-50% B; 16-20min, 0-0% B], flow rate: 0.6mL.min-1, column temperature: 30 ℃, detection wavelength: 254nm. Results: The linear range of uridine was 2.164 ~ 86.56μg, r = 0.9997. The linear range of guanosine was 2.49 ~ 99.6μg, r = 0.9999. The linear range of uricin was 3.696 ~ 147.84μg, r = 0.9999; The linear range of glucosides was 2.476 ~ 99.040μg, r = 0.9999. The average recoveries of uridine, guanosine and guanosine and adenosine were 95.9%, 99.9%, 101.0% and 99.8%, respectively, with mean RSDs of 0.8%, 1.8%, 1.3% and 0.8% ). There are differences in the contents of nucleosides in Banlangen granules of five companies. Conclusion: The method is simple, reproducible, sensitive and the results are accurate and reliable.