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Objective:To observe the effect of electroacupuncture EA on gene expression of α subunit of Go-protein in the brain of rats with hypertensive cerebral hemorrage and study its underlying mechanisms of EA in ameliorating cerebral hemorrage. Methods: A total of 130 SD rats were randomly divided into normal control group n=10, sham operation group n=40, model group n=40 and EA group n=40. The latter 3 groups were further divided into 6 h, 24 h, 48 h and 72 h time courses subgroups, with 10 rats being in each subgroup. The hypertensive cerebral hemorrage model was induced by injecting 1 μL of collagenase 0.5 U/μL collagenase Type Ⅶ and heparin 7 U/μL into the caudate nucleus in rats with renovascular hypertension by clipping the bilateral renal arteries. The gene expression of α subunit of Go-protein in the hippocampus tissue of rats was detected with Northern blotting hybridization analysis. EA continuous waves, 120 pulses/min in frequency, 1 mA in intensity and duration of 30 min was applied to "Shuigou"水沟 GV 26, bilateral "Neiguan"内关 PC 6 and bilateral "Housanli"Zusanli, 足三里 ST 36. Results: The gene expression of α subunit of Go-protein in the hippocampus tissue of the rats was obviously downregulated in hypertensive cerebral hemorrage model group and significantly upregulated after EA treatment with the extension of time. Conlusion: EA may relieve cerebral hemorrage by regulating the gene transcription of α subunit of Go-protein and increasing the expression of Go-α protein. This may be one of the molecular mechanisms of EA in improving hypertensive cerebral hemorrhage.