论文部分内容阅读
目的观察BerEP4和EMA染色在皮肤基底细胞上皮瘤和鳞状细胞癌诊断中的意义。方法用免疫组化SP法检测BerEP4和EMA在皮肤基底细胞上皮瘤、鳞状细胞癌、光线性角化病、Bowen病、脂溢性角化病、寻常疣和基底鳞状细胞癌皮损肿瘤成分及周围组织、皮肤附属腺体中的表达。结果所有基底细胞上皮瘤和基底鳞状细胞癌肿瘤细胞呈BerEP4阳性,而鳞状细胞癌、光线性角化病、Bowen病、脂溢性角化病和寻常疣呈BerEP4阴性;多数鳞状细胞癌、Bowen病和部分光线性角化病肿瘤细胞及病变区域呈EMA阳性,而基底细胞上皮瘤、基底鳞状细胞癌、脂溢性角化病和寻常疣呈EMA阴性。结论联合使用BerEP4和EMA能很好地协助诊断皮肤基底细胞上皮瘤、基底鳞状细胞癌、癌前病变及一些良性增生性皮肤病。
Objective To investigate the significance of the staining of BerEP4 and EMA in the diagnosis of basal cell carcinoma of the skin and squamous cell carcinoma. Methods Immunohistochemical SP method was used to detect the expression of BerEP4 and EMA in skin basal cell epithelial tumor, squamous cell carcinoma, photopathic keratosis, Bowen’s disease, seborrheic keratosis, verruca vulgaris and basal squamous cell carcinoma Composition and surrounding tissues, skin glands attached to the expression. Results All basal and basal squamous cell carcinoma cells were positive for BerEP4, while squamous cell carcinoma, photopathic keratosis, Bowen’s disease, seborrheic keratosis and verruca vulgaris were BerEP4 negative. Most squamous cells Bowel’s disease and some light keratosis tumor cells and lesions were EMA-positive, while basal cell carcinoma, basal squamous cell carcinoma, seborrheic keratosis and verruca vulgaris were negative for EMA. Conclusions The combination of BerEP4 and EMA can be very helpful in the diagnosis of cutaneous basal cell carcinoma, basal squamous cell carcinoma, precancerous lesions and some benign proliferative dermatoses.