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探讨外源性碱性成纤维细胞生长因子(hFGF),对缺氧缺血性脑损伤(HIBD)新生大鼠脑c-fos基因蛋白产物(c-Fos)表达影响,研究原癌基因c-fos表达与HIBD的关系,采用免疫组织化学染色及定量图像分析方法,观察c-Fos表达强度、染色灰度及面积的变化。结果显示:正常生后7-14 d新生大鼠脑有c-Fos表达,以生后10 d最为明显;缺氧缺血20 min后即刻c-Fos表达增强,并于缺氧缺血后4 h达高峰,持续至72 h;脑内不同部位c-Fos表达时间、强度、灰度及面积不同。连续腹腔注射外源性bFGF 3 d后鼠脑c-Fos表达增强。结论:外源性bFGF可增强HIBD新生大鼠脑c-Fos的表达,bFGF及c-fos基因可能参与HIBD修复的病理生理过程。
To investigate the effect of exogenous basic fibroblast growth factor (hFGF) on the expression of c-fos gene protein in neonatal rats with hypoxic-ischemic brain damage (HIBD) fos expression and HIBD, using immunohistochemical staining and quantitative image analysis methods to observe the c-Fos expression intensity, staining gray and area changes. The results showed that the expression of c-Fos in the brain of newborn rats was significantly higher than that of the normal control group (P <0.05), and the c-Fos expression was most obvious 10 days after hypoxia-ischemia. The expression of c-Fos increased immediately after hypoxia-ischemia h peaked for 72 h; different parts of the brain c-Fos expression time, intensity, grayscale and area are different. After continuous intraperitoneal injection of exogenous bFGF for 3 days, the expression of c-Fos in brain increased. Conclusion: Exogenous bFGF can enhance the expression of c-Fos in neonatal rats with HIBD. The bFGF and c-fos genes may be involved in the pathophysiological process of HIBD repair.