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目的:通过检测人乳头状瘤病毒(HPV)感染阴性的宫颈癌和宫颈上皮内瘤变中p14ARF和基质金属蛋白酶MMPs及其抑制物TIMPs的表达,探讨其与宫颈癌早期诊断、侵袭转移的关系。方法:以PCR方法筛查HPV阴性的慢性宫颈炎30例、宫颈上皮内瘤变(CIN)35例及宫颈浸润癌35例作为研究对象,采用SP染色法检测p14ARF蛋白在其中的表达,分析p14ARF蛋白在宫颈癌组织中的表达、临床病理特征及其与浸润深度、淋巴结转移的相关性。结果:p14ARF蛋白特异性表达在HPV阴性的CIN病变、宫颈癌细胞核及胞质中,在正常鳞状上皮和腺上皮中无任何阳性表达信号,且与临床分期无关,与细胞分化程度、淋巴结转移有关。结论:p14ARF蛋白检测可作为HPV阴性宫颈癌的早期诊断指标,并可作为预测宫颈癌侵袭转移潜能和临床预后的指标。
OBJECTIVE: To detect the expression of p14ARF, MMPs and their inhibitor TIMPs in human papillomavirus (HPV) -negative cervical cancer and cervical intraepithelial neoplasia, and to explore their relationship with the early diagnosis, invasion and metastasis of cervical cancer . Methods: 30 cases of HPV-negative chronic cervicitis, 35 cases of cervical intraepithelial neoplasia (CIN) and 35 cases of invasive cervical carcinoma were screened by PCR. The expression of p14ARF protein was detected by SP staining. The expression of p14ARF Protein expression in cervical cancer, clinical and pathological features and its relationship with depth of invasion, lymph node metastasis. Results: The p14ARF protein was specifically expressed in HPV-negative CIN lesions, cervical cancer cell nucleus and cytoplasm without any positive expression signal in normal squamous epithelium and glandular epithelium, and had no relation with clinical stage, with the degree of cell differentiation, lymph node metastasis related. Conclusion: The p14ARF protein can be used as an early diagnostic indicator of HPV-negative cervical cancer and can be used as an index to predict the invasion and metastasis and clinical prognosis of cervical cancer.