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无氮营养液砂培7个品种苜蓿茎叶的固氮分馏因数β值为1.0000～1.0015(δ~(15)N为-0.05～-1.47‰),白三叶、绿豆和银合欢的β值分别为0.9979、0.9983和1.0018(δ~(15)N分别为2.15、1.74及-1.81‰)。根据~(45)N天然丰度的变化,估测了田间生长苜蓿的固氮能力,表明6个品种的共生固氮能力不同。格洛里及润布勒苜蓿两品种最高,保定、阿尔贡奎因及明托苜蓿3个品种次之,秘鲁苜蓿最低。苜蓿不同时期的固氮活性有变化,6、7月间固氮活性达高峰。根据所测豆科牧草茎叶的δ~(15)N值,可以对豆科牧草进行定性或半定量水平上的固氮研究,以筛选高效固氮牧草和检测野生固氮资源。本文还对不固氮参照植物和不同方法对％Ndfa估计位的影响进行了讨论。
Nitrogenous fractionation factor β of alfalfa stem and leaves of 7 cultivars with nitrogen-free nutrient solution were 1.0000-1.0015 (-0.05 ~ -1.47 ‰ for δ ~ (15) N), β values of white clover, mung bean and albizzia 0.9979, 0.9983 and 1.0018 respectively (δ ~ (15) N were 2.15, 1.74 and -1.81 ‰, respectively). According to the change of natural abundance of ~ (45) N, the nitrogen fixation ability of alfalfa in field was estimated, indicating that the symbiotic nitrogen fixation ability of the six cultivars was different. Glori and Runbulu alfalfa the highest two varieties, Baoding, Algonquin and Mingtu alfalfa three varieties followed by Peru, the lowest alfalfa. The nitrogen fixation activity of alfalfa changed at different stages, and the nitrogen fixation activity peaked in June and July. According to the δ ~ (15) N value of the measured stems and leaves of leguminous forage grass, the nitrogen fixation of leguminous forages can be qualitatively or semi-quantitatively studied to screen efficient nitrogen fixation forages and detect the wild nitrogen fixation resources. This paper also discusses the impact of nitrogen-fixing reference plants and different methods on the% Ndfa estimated bite.