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体内外实验研究均已证明血清胸腺因子(FTS)和胸腺素α_1(THY)是促进淋巴细胞分化并增强其功能的重要因子。它们在血清中水平变化受某些生物因素和疾病的影响,诸如肿瘤、免疫缺陷或衰老等均可导致FTS、THY水平下降;此外胸腺激素在临床中的应用也很普遍,因此血清及其它体液中FTS、THY的定量分析对研究免疫系统疾病具有重要价值。 由于目前胸腺肽的生物学检测方法受体液中某些抑制因子的影响,为此我们利用邻苯二甲醛(OPA)与寡肽中ε-甘氨酸残基反应衍生较强荧光的原理,使用OPA柱前衍生法对FTS、THY测定条件进行了研究,并对血清中FTS、THY的提取及测定作了初步探讨,表明本法对体液中游离的FTS、THY定
In vitro and in vivo studies have shown that serum thymic factor (FTS) and thymosin alpha 1 (THY) is an important factor to promote lymphocyte differentiation and enhance its function. Their serum level changes are affected by certain biological factors and diseases, such as tumor, immune deficiency or aging can lead to decreased levels of FTS and THY. In addition, the clinical application of thymus hormone is also very common, and serum and other body fluids Quantitative analysis of FTS and THY is of great value in the study of immune system diseases. Because of the current thymosin biological detection methods by some of the inhibitory factors in the body fluid, we use the phthalaldehyde (OPA) oligopeptide epsilon-glycine residues derived stronger fluorescence principle, the use of OPA column Pre-derivatization method FTS, THY determination of the conditions were studied, and serum FTS, THY extraction and determination made a preliminary study shows that the body fluid free FTS, THY set