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使用Sephadex G-75注入家兔颈内动脉制造缺血性脑血管疾病的模型,取出脑组织,分离微粒体,加入~(14)C标记的花生四烯酸,将反应后产生的各种前列腺素(PGs)分离,计算其生成率。发现对照组中左右各脑组织中的前列腺素生成无差异,在大脑白质以外的部位前列腺素PGF_(2α)产生最多,6-keto-PGF_(1α)产生最少,大脑白质中PGD_2,TXB_2及PGE_2较其它部位产生的多。脑缺血组的前列腺素代谢除了PGD_2以外均较对照组高,且阻塞侧高于非阻塞侧。PGF_(2α)的代谢在缺血后无左右差。此方法制造脑缺血可靠性大,且侵袭性较小,比较适合进行前列腺素代谢的测定。
A model of ischemic cerebrovascular disease was injected into the carotid artery of rabbits by injecting Sephadex G-75 into the carotid artery of the rabbits. The brain tissue was removed and the microsomes were isolated. A ~ (14) C-labeled arachidonic acid was added and the various prostate (PGs) were separated and the rate of formation was calculated. The results showed that there was no difference in the prostaglandin production between the left and right brain tissues in the control group, the highest PGF_ (2α) production, the lowest 6-keto-PGF_ (1α) production, the PGD_2, TXB2 and PGE_2 in the white matter More than other parts of the generated. In addition to PGD_2, prostaglandin metabolism in cerebral ischemic group was higher than that in control group, and occlusion side was higher than non-occlusion side. Metabolism of PGF_ (2α) did not change after ischemia. This method has the advantages of high reliability of cerebral ischemia and less invasiveness, and is more suitable for the determination of prostaglandin metabolism.