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目的本研究通过对黄连种子萌发及幼苗生理特性的研究,旨在寻找提高黄连种子及幼苗在盐胁迫条件下抗性能力的途径。方法用100 mmol/L的Na Cl模拟盐胁迫,在外源5-氨基乙酰丙酸(ALA)处理后,对黄连种子的各萌发指标及黄连幼苗叶片细胞质膜透性、超氧阴离子产生速率,过氧化氢(H2O2)量,可溶性糖、可溶性蛋白、游离脯氨酸及丙二醛(MDA)量、超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)和抗坏血酸过氧化物酶(APX)活性进行测定。结果 Na Cl胁迫下的黄连种子萌发受到显著抑制,但是在经过不同浓度的ALA处理后,发芽势、发芽率、发芽指数和活力指数等萌发指标均有升高。外源ALA处理显著提高了盐胁迫下黄连幼苗叶片可溶性糖、可溶性蛋白和脯氨酸量,不同程度的提高了叶片超SOD、POD和CAT活性,显著降低了MDA量、质膜透性、超氧阴离子产生速率及H2O2量。结论外源ALA通过提高黄连种子的萌发指数,提高渗透调节物质含量及抗氧化酶活性,降低细胞质膜氧化程度,有效地减缓Na Cl胁迫对黄连种子及幼苗产生的伤害,提高了种子及幼苗的抗盐能力。
Aim To find the ways to improve the resistance of Coptis chinensis seeds and seedlings under salt stress by studying the seed germination and seedling physiological characteristics of Coptis chinensis. Methods 100 mmol / L NaCl was used to simulate salt stress. After treatment with exogenous 5-aminolevulinic acid (ALA), the germination indexes of Coptis chinensis seeds and the plasma membrane permeability, superoxide anion production rate (H2O2), soluble sugar, soluble protein, free proline and malondialdehyde (MDA), superoxide dismutase (SOD), peroxidase (POD), catalase (CAT) And ascorbate peroxidase (APX) activity were determined. Results The seed germination of Coptis chinensis under NaCl stress was significantly inhibited. However, germination potential, germination rate, germination index and vigor index increased after treatment with different concentrations of ALA. Exogenous ALA treatment significantly increased the content of soluble sugar, soluble protein and proline in leaves of Coptis chinensis seedling under salt stress, and increased the activities of superoxide dismutase, peroxidase and peroxidase, and significantly decreased the contents of MDA and membrane permeability Oxygen anion generation rate and amount of H2O2. Conclusion Exogenous ALA could improve the content of osmotic adjustment substances and the activity of antioxidant enzymes, decrease the level of plasma membrane oxidation and alleviate the damage caused by NaCl stress to the seeds and seedlings of Coptis chinensis by improving the germination index of Coptis chinensis seeds, Salt resistance.