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七十年代束,Towhin等[1]设计了一种将聚丙烯酰胺凝胶电泳(PAGE)、电泳转移和免疫标记方法结合而成的免疫学新技术——免疫印迹怯(Immunoblot,又称Westernblot),大大推动了寄生虫生物学和免疫化学的进展。本文就免疫印迹法的一般原理、方法及其在寄生虫免疫学中的应用作一概述。 一、原理SDS-PAGE对抗原分离具有高的分辨率,并能确定抗原组分的分子量。有人用薄层等电聚焦(IEF)和琼脂糖电泳,以避免SDS对抗
In the 1970s, Towhin et al. [1] designed a novel immunological technique combining polyacrylamide gel electrophoresis (PAGE), electrophoretic transfer and immunolabeling - Immunoblot (also known as Western blot ), Has greatly promoted the progress of parasite biology and immunochemistry. This article summarizes the general principles and methods of immunoblotting and their applications in parasite immunology. First, the principle of SDS-PAGE antigen separation with high resolution, and to determine the molecular weight of antigen components. Some people use thin-layer isoelectric focusing (IEF) and agarose electrophoresis to avoid SDS confrontation