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诱导型多能干细胞(induced pluripotent stem cells,iPS cells)技术的建立为自体组织工程治疗带来了新的希望。鉴于糖尿病患者常伴有骨再生性障碍,该研究比较了不同葡萄糖浓度下小鼠iPS细胞的成骨能力,并探讨了骨形态蛋白4(bone morphogenetic protein 4,BMP4)在该过程中的作用。实验结果显示:成骨诱导21 d后,低糖组茜素红阳性细胞比例和成骨基因Runx2、Osteocalcin的表达水平显著高于高糖组和自发分化组(P<0.05);BMP4的添加提高了高糖组茜素红阳性细胞比例及Osteocalcin的表达水平(P<0.05),而对自发分化组细胞的成骨水平无影响。该结果表明:低葡萄糖含量对小鼠iPS细胞的骨向分化有促进作用,尽管BMP4可以提高高糖组小鼠iPS细胞的成骨能力,但仅在成骨分化条件下发挥作用。
The establishment of induced pluripotent stem cells (iPS cells) technology has brought new hope for autologous tissue engineering. Since diabetics are often associated with bone-regenerative disorders, this study compared the osteogenic potential of mouse iPS cells at various glucose concentrations and explored the role of bone morphogenetic protein 4 (BMP4) in this process. The results showed that the proportion of alizarin red positive cells and the expression of Runx2 and Osteocalcin in low glucose group were significantly higher than those in high glucose group and spontaneous differentiation group after 21 days osteogenic induction (P <0.05); BMP4 addition increased The ratio of alizarin red positive cells and the expression of Osteocalcin in high glucose group (P <0.05), but had no effect on the osteogenic level of spontaneous differentiation cells. The results showed that low glucose could promote the osteogenic differentiation of iPS cells. Although BMP4 could enhance the osteogenic ability of iPS cells in high glucose group, it only played a role in osteogenic differentiation.