论文部分内容阅读
以我国重要香蕉种质‘过山香’(AAB)为材料,研究了香蕉多芽体诱导、多芽体茎尖薄切片即分生组织性的表层结构物(scalp)愈伤组织诱导和体细胞胚发生的合适条件。结果表明,该品种单个不定芽茎尖在P4培养基(含BAP100μmol·L-1和IAA1μmol·L-1)中继代5个周期后可诱导获得类似花椰菜结构的多芽体。从30μmol·m-2·s-1光强,光/暗周期(16h/8h)培养的多芽体所获得的scalp在添加24D5μmol·L-1和Zeatin1μmol·L-1的愈伤组织诱导培养基中黑暗培养,45d后愈伤组织诱导率可达97.6%。诱导20d后黄色分生小球体类结节状愈伤组织开始发生,90～120d后在分生小球体局部可获得白色或浅黄色松散的胚性愈伤组织(诱导率为17.5%)。从胚性愈伤组织诱导的体细胞胚在成熟培养基上培养60d后体胚萌发率和植株转化率分别为14.5%和11.1%
A banana germplasm (AAB) was used as material to study the induction of callus from banana shoots and the induction of the meristem scalp callus and somatic cells Suitable conditions for embryogenesis. The results showed that the shoot tips of single adventitious bud of this variety could induce multi-bud similar cauliflower structure after subculture of P4 medium (containing BAP 100μmol·L-1 and IAA1μmol·L-1) for 5 cycles. Scalp obtained from the spores of 30μmol · m-2 · s-1 light intensity and light / dark cycle (16h / 8h) were cultured in callus induced by adding 24D5μmol·L-1 and Zeatin1μmol·L-1 In the dark medium, the callus induction rate reached 97.6% after 45 days. After 20 days of induction, the nodular callus of yellow meristem began to occur. After 90 ~ 120 days, white or light yellow loose embryogenic callus could be obtained locally (induced rate was 17.5%). Somatic embryos induced from embryogenic callus on maturation medium for 60 days after germination and plant transformation rate of 14.5% and 11.1%