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Objective: To observe the curative effect of the traditional Chinese medicine (TCM) Qing Yi Tang (QYT, 清胰汤) in acute necrotizing pancreatitis (ANP). Methods: Twenty three dogs were randomly divided into 3 groups. In the control group (n = 7), animals underwent laparatomy only. In the ANP group (n =8), acute necrotizing pancreatitis was induced by injection of 0. 5 ml/kg 5 % sodium taurocholate with 3000 u/kg trypsin into the pancreatic duct. While in TCM group (n = 8) were fed everyday with QYT after onset of ANP. All animals were sacrificed 7 days later and organs were gathered and cultured. Mucosal and luminal floras of the intestine were analysed. Pancreas and ileal mucosa were examined histologically and ultramicroscopically, the levels of lipopolysaccharide (LPS) and amylase in blood were determined. Results: In the TCM group, histologic and ultra-structural damages in pancreas and lieal mucosa were much milder as compared with those of ANP group. In ANP group, there was a significant increase of E. colt and bacteroids, and a significant decrease of bifidobacteria, lactobacilli and enterococci in the intestinal mucosa, while in the TCM group, these changes were alleviated significantly (P< 0. 05 or P < 0. 01 ). As compared with the ANP group, the bacterial translocation (BT) rate was reduced from 100 % to 50 %, and the counts of translocated bacteria were decreased 10 - 40 times, the levels of LPS and amylase reduced 2 - 3 times. Conclusions: TCM recipe QYT showed their protective effects on gut barrier function by alleviating the damage of intestinal mucosa and microecologic disturbance following acute pancreatitis. As a result, the chances of BT and enterogenic infection declined. These preparation might be promising in the prophylaxis and treatment of infection complicating ANP.
Objective: To observe the curative effect of the traditional Chinese medicine (TCM) Qing Yi Tang (QYT, 清胰汤) in acute necrotizing pancreatitis (ANP). Methods: Twenty three dogs were randomly divided into 3 groups. In the control group ( n = 7), animals underwent laparatomy only. In the ANP group (n =8), acute necrotizing pancreatitis was induced by injection of 0. 5 ml/kg 5% sodium taurocholate with 3000 u/kg trypsin into the pancreatic duct. While In TCM group (n = 8) were fed everyday with QYT after onset of ANP. All animals were sacrificed 7 days later and organs were gathered and cultured. Mucosal and luminal floras of the intestine were analysed. Pancreas and ileal mucosa were examined histologically and Ultramicroscopically, the levels of lipopolysaccharide (LPS) and amylase in blood were determined. Results: In the TCM group, histologic and ultra-structural damages in pancreas and lieal mucosa were much milder as compared with those of ANP group. In ANP group, there Was as Genicificant increase of E. colt and bacteroids, and a significant decrease of bifidobacteria, lactobacilli and enterococci in the intestinal mucosa, while in the TCM group, these changes were alleviated significantly (P< 0. 05 or P < 0. 01 ). As Compared with the ANP group, the bacterial translocation (BT) rate was reduced from 100% to 50%, and the counts of translocated bacteria were decreased 10 - 40 times, the levels of LPS and amylase reduced 2 - 3 times. Conclusions: TCM Approach QYT showed their protective effects on gut barrier function by alleviating the damage of intestinal mucosa and microecologic disorder the requirement of acute pancreatitis. As a result, the chances of BT and enterogenic infection declined. These preparation might be promising in the prophylaxis and treatment of infection complicating ANP.