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目的 :建立获得性多药耐药 (MDR)表达小鼠腹水模型 ,探讨检测S180细胞和外周血淋巴细胞MDR1表达水平及凋亡指标的意义。方法 :给予S180细胞荷瘤小鼠反复定量联合化疗刺激 ,用流式细胞术动态检测S180细胞和同期外周血淋巴细胞MDR1表达水平及凋亡指标结果 :( 1)实验组小鼠S180自化疗后 1周MDR1表达逐渐增高 ,第 5周后显著增加 (P <0 .0 5) ;而细胞凋亡率、细胞杀伤率及Fas因子表达率则随化疗时间的延长而逐渐下降 ,且MDR1表达显著增加时 ,S180细胞凋亡率等则显著降低 (P <0 .0 5) ;( 2 )实验组外周血淋巴细胞MDR1、细胞凋亡水平与S180细胞显著相关。结论 :小鼠S180细胞多药耐药模型有助于获得在体研究MDR1表达的经验 ,经数疗程化疗后外周血淋巴细胞MDR1表达能够反映S180细胞MDR1表达的水平
Objective: To establish an ascitic fluid model of acquired multidrug resistance (MDR) in mice and to explore the significance of MDR1 expression and apoptosis in S180 cells and peripheral blood lymphocytes. Methods: S180 tumor-bearing mice were treated with repeated quantitative chemotherapy combined with chemotherapy. Flow cytometry was used to detect the expression of MDR1 in S180 cells and peripheral blood lymphocytes in the same period. Results: (1) The expression of MDR1 gradually increased at 1 week, and increased significantly after 5 weeks (P <0.05). However, the apoptosis rate, cell killing rate and the expression of Fas factor decreased with the prolongation of chemotherapy time, and the expression of MDR1 was significant (P <0.05). (2) The levels of MDR1 and apoptosis in peripheral blood lymphocytes in experimental group were significantly correlated with S180 cells. CONCLUSIONS: The multidrug resistance model of mouse S180 cells is helpful to obtain MDR1 expression in vivo. MDR1 expression in peripheral blood lymphocytes can reflect the level of MDR1 expression in S180 cells after several cycles of chemotherapy