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为探索基于老龄大鼠建立“Ⅱ型”糖尿病模型的方法。该研究纳入12只12月龄雄性Wistar大鼠,在大鼠连续4个月自由采食高脂饲料后,给予3次腹腔注射低剂量链脲佐菌素溶液(剂量为20 mg/kg,浓度为10 mg/m L),并检测给药前后大鼠空腹血糖浓度数据,将空腹血糖浓度高于11.1 mmol/L的大鼠定为“Ⅱ型”糖尿病模型。实验结果显示,大鼠自由采食高脂饲料4个月后,体重由604.8±13.7 g增加为760.6±31.6 g(P<0.01);连续3次注射低剂量链脲佐菌素溶液后,空腹血糖浓度由5.6±0.7 mmol/L增加为22.9±2.7 mmol/L(P<0.01);继续观察2周后,大鼠空腹平均血糖浓度为17.4 mmol/L,仍显著高于糖尿病诊断标准的空腹血糖最低值11.1 mmol/L(P<0.01),糖尿病模型制备的成功率为83.3%。结果表明,连续注射3次低剂量链脲佐菌素溶液并结合高脂饲料饲养,可成功将老年Wistar大鼠诱导为“Ⅱ型”糖尿病模型。
To explore the method of establishing “type II” diabetes model in aged rats. Twelve male Wistar rats, aged 12 months, were enrolled in this study. After the rats were fed with high-fat diet freely for 4 months, they were given intraperitoneal injection of low-dose streptozotocin solution (20 mg / kg, 10 mg / m L). The fasting blood glucose levels of rats before and after administration were measured. The rats with fasting blood glucose levels higher than 11.1 mmol / L were classified as type II diabetes. The results showed that body weight increased from 604.8 ± 13.7 g to 760.6 ± 31.6 g after 4 months (P <0.01). After three consecutive injections of low-dose streptozotocin solution, fasting Blood glucose concentration increased from 5.6 ± 0.7 mmol / L to 22.9 ± 2.7 mmol / L (P <0.01). After two weeks of continuous observation, the fasting mean blood glucose level in rats was 17.4 mmol / L, which was still significantly higher than the diagnostic criteria of diabetes fasting The lowest blood glucose level was 11.1 mmol / L (P <0.01), and the success rate of diabetic model preparation was 83.3%. The results showed that the Wistar rats could be successfully induced to “type Ⅱ” diabetes mellitus by continuous injection of 3 doses of low-dose streptozotocin solution combined with high-fat diet.