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目的探讨卵巢上皮性癌（简称卵巢癌）肿瘤浸润淋巴细胞（ｔｕｍｏｒｉｎｆｉｌｔｒａｔｉｎｇｌｙｍｐｈｏｃｙｔｅｓ，ＴＩＬ）的生物学特性。方法用机械法和酶法消化实体瘤后，采用分步低速离心后行连续密度梯度法分离卵巢癌ＴＩＬ，在有ＩＬ２、ＰＨＡ或ＯＫＴ３条件下培养，表型分析采用间接免疫荧光染色法，细胞毒性检测采用ＭＴＴ法。结果１５份卵巢癌ＴＩＬ中，得率范围１０６～１０７／ｇ，１３份ＴＩＬ活化（另２份污染），最大扩增倍数５２３倍，其中７份ＴＩＬ经培养达治疗量（１０９个细胞）；初分离的卵巢癌ＴＩＬ处于功能抑制状态，抑制期５～１５天，随着活化后体外培养，ＣＤ３、ＣＤ８、ＣＤ２５（ＩＬ２Ｒ）和ＨＬＡＤＲ表达显著增多；初分离的卵巢癌ＴＩＬ对Ｋ５６２和自体瘤细胞杀伤力均低下，随着体外培养，细胞毒性增加，尤以对自体瘤的特异性杀伤力增加最显著。结论卵巢癌ＴＩＬ体外易分离及活化，特异性杀伤力较强，适于肿瘤的生物学治疗
Objective To investigate the biological characteristics of tumorinfiltrating lymphocytes (TIL) in epithelial ovarian cancer (Ovarian cancer). Methods After solid tumors were digested by mechanical method and enzymatic method, the TILs of ovarian cancer were separated by continuous low-speed centrifugation followed by stepwise low-speed centrifugation and cultured in IL2, PHA or OKT3. The phenotypes were analyzed by indirect immunofluorescence staining , Cytotoxicity detection using MTT method. Results The yield of 10 cases of TIL in ovarian cancer was 106-107 / g, 13 TILs were activated (the other 2 were contaminated) and the maximum amplification was 523 fold. Among them, 7 TILs were treated with 109 cells. TIL ovarian cancer initially isolated in a functional inhibition of inhibition of 5 to 15 days, with the activation of cultured in vitro, CD3, CD8, CD25 (IL 2R) and HLA DR expression was significantly increased; K562 and autologous tumor cells were low in lethality, with in vitro culture, increased cytotoxicity, especially for autologous tumor-specific cytotoxicity increased the most significant. Conclusion Ovarian TIL is easy to separate and activate in vitro with strong specific killing ability and is suitable for biological treatment of tumors