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在美国西北部,采用常规方法进行牡蛎三倍体生产,往往会导致美国牡蛎(Crassostrea virginica)90%以上的死亡率,这个高比例在东海岸的大多数孵化场是不能接受的.我们就细胞松弛素B(CB)浓度、受精后的处理时间及处理持续时间对美国牡蛎胚胎的成活及三倍体诱导的影响进行了试验.结果表明D形幼虫成活率与CB浓度及其处理时间成反比.三倍体率则取决于CB剂量.当半数胚胎发育至第一次减数分裂时进行0.25mg/L CB处理10～15min可获得平均成活率为对照组84%的D形幼虫,而相应的三倍体率可达96%.
In the northwestern United States, conventional methods of oyster triploiding often result in more than 90% mortality in U.S.sssss (Crassostrea virginica), a high percentage of most hatcheries on the east coast. The concentration of CB, the treatment time after fertilization and the duration of treatment on the survival and triploid induction of American oyster embryos were tested.The results showed that the survival rate of D-shaped larvae was inversely proportional to CB concentration and its treatment time .Thypothesis rate depends on CB dose.When half of the embryos developed to the first meiosis 0.25mg / L CB treatment 10 ~ 15min average survival rate of 84% of the control group D larvae, and the corresponding Triploidy rate up to 96%.