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目的研究一株海洋放线菌Streptomyces sp.(No.30701)的化学成分。方法采用硅胶开放柱色谱、Sephadex LH-20凝胶柱色谱以及高效液相色谱等分离手段进行化合物的分离、纯化;利用理化性质和波谱学分析对单体化合物进行结构鉴定。结果从海洋放线菌Streptomyces sp.(No.30701)发酵物中分离得到9个环二肽类化合物,分别鉴定为环(L-脯-L-缬)二肽(1)、环(D-脯-L-缬)二肽(2)、环(L-脯-L-亮)二肽(3)、环(4-羟基-脯-亮)二肽(4)、环(L-脯-L-异亮)二肽(5)、环(L-脯-L-酪)二肽(6)、环(L-亮-L-缬)二肽(7)、环(D-苯丙-甘)二肽(8)、环(L-苯丙-L-缬)二肽(9)。结论以上化合物均为海洋放线菌常见的次生代谢产物,但化合物2、8含有天然界中不多见的D型氨基酸,并且化合物2是从链霉菌属放线菌中首次分离得到。
Objective To study the chemical constituents of a marine actinomycete Streptomyces sp. (No.30701). Methods The compounds were isolated and purified by silica gel column chromatography, Sephadex LH-20 column chromatography and high performance liquid chromatography (HPLC). The structures of the compounds were identified by physicochemical and spectroscopic methods. Results Nine cyclic dipeptides were isolated from the marine actinomycetes Streptomyces sp. (No.30701) and identified as dipeptide (1), D- (2), cyclic (L-pro-L-light) dipeptide (3), cyclohepta-4-hydroxy- L-isoleucyl) dipeptide (5), cyclic (L-lactam-L-lactide) dipeptide (6) Gly) dipeptide (8), cyclo (L-phenylpropionyl) -L-valine dipeptide (9). CONCLUSION The above compounds are common secondary metabolites of Actinomycetes, but compounds 2 and 8 contain D-type amino acids, which are rare in nature. Compound 2 was isolated from Streptomyces actinomycetes for the first time.