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目前,GH的诊断主要依靠临床诊断,但对于非典型GH患者仍需借助实验室方法,作为GH诊断的“金标准”病毒培养法,以及其它如 DNA分子原位杂交法、免疫印迹法等则多由于操作技术要求过高而不适于临床应用。近年来,PCR法,酶免疫法开始应用于临床,PCR检测皮损HSV核酸敏感性和特异性高,能大大提高生殖器溃疡病人中HSV的确证能力。最近我院又开展了HSV抗原ELISA检测法,为评价这几种实验室诊断方法,并探讨GH的临床特征,我们对 84例临床诊断为 GH患者做了 PCR、HSV抗原ELISA检测及血清学检测,并探讨GH的临床感染
Currently, the diagnosis of GH depends mainly on clinical diagnosis, but for atypical GH patients still need laboratory methods, as the GH diagnosis of “gold standard” virus culture and other methods such as DNA in situ hybridization, Western blot More due to the technical requirements of the operation is not suitable for clinical applications. In recent years, PCR, enzyme immunoassay began to be used clinically, PCR detection of skin lesions HSV nucleic acid sensitivity and specificity, can greatly enhance genital ulcer patients confirmed the ability of HSV. Recently, our hospital also launched HSV antigen ELISA detection method, to evaluate these types of laboratory diagnostic methods, and to explore the clinical features of GH, 84 cases of clinical diagnosis of GH patients with PCR, HSV antigen ELISA and serological tests , And to explore the clinical implications of GH