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目的 探讨pLXSN-Tum-5病毒颗粒对人脐静脉内皮细胞(HUVEC)的生长抑制和诱导凋亡作用及机制.方法 采用不同浓度的pLXSN-Tum-5病毒颗粒作用HUVEC,利用噻唑蓝法和Hoechst-33342染色法检测细胞生长及其形态变化;RT-PCR和Western blotting方法检测细胞中Bax、Bcl-2、caspase-3 mRNA和蛋白表达变化.结果 与对照组(pLXSN)比较,pLXSN-Tum-5病毒颗粒转染组HUVEC的存活率明显降低,呈剂量效应关系;细胞内可见凋亡小体;对照组HUVEC内Bcl-2和caspase-3 mRNA和蛋白表达分别为(0.721 ±0.041)、(0.654±0.034)和(0.956±0.032)、(0.356±0.054);与对照组比较,20 μmol/L pLXSN-Tum-5病毒颗粒转染组HUVEC内Bcl-2和caspase-3 mRNA和蛋白水平[分别为(1.134±0.0524)、(1.012±0.0641)和(1.612 ±0.067)、(0.712±0.0647)]明显上调(P≤0.05).结论 Tum-5可抑制HUVEC增殖,诱导HUVEC凋亡,其机制可能与上调Bax/Bcl-2和caspase-3表达有关.“,”Objective To explore growth inhibition and apoptosis induction effects of pLXSN-Tum-5 in human umbilical vein endothelial cells (HUVEC) and the mechanism of the effects.Methods HUVEC were treated with pLXSN-Tum-5 virus particles at different concentrations.Hoechst-33342 staining and 3-(4,5)-dimethylthiahiazo (-z-yl)-3,5-diphenytetrazoliumromide (MTT) assay were utilized to monitor the growth states and morphological changes of HUVEC.Changes in the expressions of intracellular B cell lymphoma 2 (Bcl-2),Bcl-2-associated X (Bax),and caspase-3 at mRNA and protein levels were determined with real-time reverse transcription PCR (RT-PCR) and Western blot.Results The survival rate of HUVEC transfected with pLXSN-Tum-5 virus particles was significantly decreased in a dose-effect manner compared to that of the control (with pLXSN).Apoptotic bodies were observed in the HUVEC transfected with pLXSN-Tum-5.Significantly upregulated mRNA and protein expressions of Bcl-2 (1.134 ± 0.0524 vs.0.721 ± 0.041and 1.012 ± 0.0641 vs.0.654 ± 0.034) and caspase-3 (1.612--0.067 vs.0.956 ± 0.032 and 0.712 ± 0.0647 vs.0.356 ±0.054) were observed in the HUVEC transfected with 20 mol/L pLXSN-Tum-5 virus particles compared to those in the control HUVEC (P ≤ 0.05 for all).Conclusion Human Tum-5 could inhibit proliferation and induce apoptosis in HUVEC,and the mechanism of the effects may be related to upregulated expressions of Bax/Bcl-2 and caspase-3 in the