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目的改进双环己铜草酰二腙(cuprizone,CPZ)诱导脱髓鞘小鼠模型的制备方法,为治疗脱髓鞘疾病提供更精准快速的动物模型。方法取60只18~20 g的C57BL/6小鼠,分成空白对照组,CPZ灌胃组(共4组,300 mg·kg~(-1),qd,bid,tid,qid),CPZ饲喂组(即传统模型组),每组10只。空白对照组及CPZ灌胃组给予正常饲料,CPZ饲喂组给予0.2%CPZ混合鼠饲料连续饲喂6周构建小鼠脱髓鞘模型,通过免疫组化和髓鞘染色技术LFB检测髓鞘脱失情况,并比较CPZ灌胃模型和传统模型诱导小鼠急性髓鞘脱失的差异。结果作用3周后,4组CPZ灌胃组的小鼠脑胼胝体区髓鞘碱性蛋白MBP染色与对照组相比均明显减少,而传统模型中小鼠脑胼胝体区MBP在第3周并未显著改变,在第6周明显减少。LFB染色结果表明CPZ灌胃3周后小鼠脑胼胝体区染色显著减少。结论改进后的模型(小鼠CPZ灌胃模型)小鼠在第3周即能出现脱髓鞘现象,能有效缩短时间,具有显著优越性,且CPZ剂量可控,保证模型的精准性。
Objective To improve the preparation of demyelination mouse model induced by cuprizone (CPZ) and provide a more accurate and rapid animal model for the treatment of demyelinating diseases. Methods 60 C57BL / 6 mice (18-20 g) were divided into blank control group, CPZ group (4 rats in total, 300 mg · kg -1, qd, bid, tid, qid) Feeding group (ie, the traditional model group), each group of 10. The blank control group and CPZ gavage group were given normal diet. The CPZ fed group was given 0.2% CPZ mixed rat feed for 6 weeks to construct the mouse demyelination model. The myelin was detected by immunohistochemistry and myelin staining And compare the difference between CPZ-fed mice model and traditional model induced acute demyelination in mice. Results After 3 weeks of treatment, the MBP staining of myelin basic protein in the corpus callosum of four groups of CPZ-treated mice was significantly decreased compared with that of the control group, while the MBP of the mouse corpus callosum area in the traditional model did not show significant difference at the third week Change, significantly reduced in the 6th week. The results of LFB staining showed that the staining of corpus callosum in mice was significantly reduced after 3 weeks of CPZ administration. Conclusion The improved mouse model of CPZ administration can demyelination in the third week, which can effectively shorten the time and has significant advantages. The dose of CPZ can be controlled to ensure the accuracy of the model.