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用桔梗茎段为外植体,以MS为基本培养基,对添加不同浓度和比例的外源激素对桔梗丛生芽的诱导、增殖及植株再生的影响进行探讨。结果表明:丛生芽诱导培养基以MS+6-BA0.3mg/L+NAA0.05mg/L效果最佳;增殖培养基以MS+6-BA2.0mg/L+NAA0.5mg/L效果最佳;生根培养基以1/2MS+NAA0.2mg/L效果最佳。
Using the stem segments of Campanulaceae as explants and MS as the basic medium, the effects of exogenous hormones with different concentrations and ratios on the induction, proliferation and plant regeneration of Campanulaceous buds were studied. The results showed that the effect of MS + 6-BA0.3mg / L + NAA0.05mg / L was the best in clustered shoot induction medium and the best medium was MS + 6-BA2.0mg / L + NAA0.5mg / L ; Rooting medium to 1 / 2MS + NAA0.2mg / L best.