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目的为探索杭州市空气中细颗粒物微生物的分布特点,采集夏秋两季5个代表性区域里的空气PM2.5微生物样本,分析微生物的数量和种类。方法 Andersen空气微生物采样器采集PM2.5微生物样本,分别进行微生物计数,采用传统培养鉴定方法、全自动细菌鉴定仪和普通PCR方法分别进行霉菌、细菌和分枝杆菌属细菌的鉴定。结果夏秋两季空气PM2.5细菌和霉菌数量商业区最多。β溶血细菌交通枢纽区最多。分离到10属细菌,优势菌为芽胞杆菌属;分离到12属霉菌,优势菌为曲霉属。仅从商业区样本中扩增到分枝杆菌属16S rRNA的保守区基因片段。结论商业区空气细颗粒物微生物污染最严重,其次分别是交通枢纽区、居民区、高校教学区和风景区。
Objective To explore the distribution characteristics of fine-grained microorganisms in the air of Hangzhou City, and to collect the PM2.5 airborne microorganisms samples from 5 representative areas in summer and autumn, and to analyze the number and types of microbes. Methods Andersen air microbial sampler was used to collect PM2.5 microbiological samples. The microorganisms were counted respectively. The bacteria, bacteria and Mycobacterium were identified by traditional culture identification method, automatic bacterial identification instrument and ordinary PCR method respectively. The results of summer and autumn air PM2.5 bacterial and mold number commercial area up. β hemolytic bacteria transport hub up to. Ten bacteria were isolated and the dominant bacteria were Bacillus. Twelve molds were isolated and the dominant bacteria were Aspergillus. The conserved region gene fragment of Mycobacterium 16S rRNA was amplified from commercial samples only. Conclusion The commercial air pollution by airborne particulate matter is the most serious, followed by transportation hub, residential area, college teaching area and scenic area respectively.