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对各丙型肝炎病毒(HCV)分离株序列分析表明,至少存在12个基因型。现在看来,HCV感染的致病性、对IFN-α治疗的反应、HCV RNA的水平及其慢性感染的临床过程似乎都与HCV基因型部分相关。目前用RT-PCR方法进行基因分型有复杂、昂贵和费时等问题。Tsukiyama-Kohara等发现,根据HCV NS3～4氨基酸序列和系统发生分析结果,可将大部分日本HCV感染株分成1型和2型两种类型,且这一区域的氨基酸序
Sequence analysis of each Hepatitis C virus (HCV) isolate showed that there were at least 12 genotypes. It now appears that the pathogenicity of HCV infection, the response to IFN-a therapy, the level of HCV RNA, and the clinical course of its chronic infection appear to be partly related to the HCV genotype. The current genotyping by RT-PCR method is complicated, expensive and time consuming. Tsukiyama-Kohara et al. Found that based on the HCV NS3 amino acid sequence and phylogenetic analysis, most Japanese HCV strains can be divided into two types, type 1 and type 2, and the amino acid sequence of this region