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本文研究报道了抗稻瘟病细胞突变体筛选方法、程序与结果,并对有关问题进行了讨论。毒素对水稻成熟胚愈伤组织的诱导与分化有明显的抑制作用,抑制程度随毒素浓度提高和处理时间延长而加强,但抗感品种之间差异显著。以含毒素的诱导培养基或诱导与分化培养基均含毒素的筛选处理效果较好,而且简便易行。5年来接种筛选了29个品种25000多个外植体,在已鉴定的349个R_1个体中,有183株表现抗病。经多次接种鉴定与选择,已获得12个高抗稻瘟病的株系和1个抗病丰产品系,并对部分抗病突变体的抗性基因进行了遗传分析。
In this paper, we reported the screening methods, procedures and results of blast-resistant cell mutants, and discussed the related issues. Toxins significantly inhibited the induction and differentiation of callus from mature embryos in rice, and the degree of inhibition was enhanced with the increase of toxin concentration and the prolongation of treatment time, but there was significant difference among resistant varieties. Toxin-containing induction medium or induction and differentiation medium containing toxins screening effect is better, and simple and easy. Over the past five years, more than 25,000 explants from 29 cultivars were screened and out of 349 R_1 individuals identified, 183 showed disease resistance. After multiple inoculation identification and selection, 12 lines with high blast resistance and 1 line with high disease resistance have been obtained, and some resistance genes of some disease-resistant mutants have been analyzed by genetic analysis.