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目的:检测乳腺癌患者外周血T淋巴细胞亚群及NK、NKT细胞表达率的变化,探讨乳腺癌患者免疫状况与肿瘤分期的关系以及免疫指标变化在治疗前后的临床价值。方法 :采用荧光标记法与多参数流式细胞仪检测40例正常女性和120例乳腺癌患者的总T淋巴细胞(CD3+)、T辅助/T诱导细胞(CD3+CD4+)、T抑制/细胞毒细胞(CD3+CD8+)、自然杀伤细胞(NK)、NK样T细胞(NKT)的表达率,并进行统计学分析。结果:无淋巴结转移组乳腺癌患者治疗前CD3+、CD3+CD4、CD3+CD8+、CD4+/CD8+与对照组比较无统计学意义,NK、NKT显著低于对照组(P<0.05)。淋巴结转移组乳腺癌患者CD3+、CD3+CD4、CD3+CD8+、NK、NKT表达率均明显低于对照组(P<0.05)。经过综合治疗后乳腺癌组各项免疫指标均恢复到正常值。结论:外周血T细胞亚群的检测对判断乳腺癌患者的病情、临床分期有一定意义;经过治疗后能够有效地改善乳腺癌患者的免疫状态。
Objective: To detect the changes of T lymphocyte subsets, NK and NKT cells in peripheral blood of patients with breast cancer, and to investigate the relationship between immune status and tumor stage in patients with breast cancer and the clinical value of immune index changes before and after treatment. Methods: The total T lymphocyte (CD3 +), T helper / T induced cells (CD3 + CD4 +), T suppressor / cytotoxicity in 40 normal women and 120 breast cancer patients were detected by fluorescence labeling and multiparameter flow cytometry (CD3 + CD8 +), natural killer (NK) and NK-like T lymphocyte (NKT) were detected and analyzed statistically. Results: The levels of CD3 +, CD3 + CD4, CD3 + CD8 + and CD4 + / CD8 + in patients with breast cancer without lymph node metastasis before treatment were significantly lower than those in control group. NK and NKT were significantly lower than those in control group (P <0.05). The expression of CD3 +, CD3 + CD4, CD3 + CD8 +, NK and NKT in breast cancer patients with lymph node metastasis was significantly lower than that in control group (P <0.05). After the comprehensive treatment of breast cancer group of immune indicators were restored to normal. Conclusion: The detection of T lymphocyte subsets in peripheral blood has certain significance for judging the condition and clinical stage of breast cancer patients. After treatment, the immune status of breast cancer patients can be effectively improved.