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目的:研究丹皮酚(Paeonol,Pae)对人胶质母细胞瘤细胞增殖的影响及其对化疗药物敏感性的影响。方法:应用水溶性四唑盐法,检测不同浓度和时间点Pae对体外培养人胶质母细胞瘤U251细胞增殖的影响,联合Pae及不同浓度依托泊苷(VP-16),检测Pae对VP-16细胞毒性的影响并以SPSS17.0系统软件计算IC50值。结果:(1)Pae可呈剂量依赖性抑制人胶质母细胞瘤U251细胞的增殖,Pae在浓度为15.63 mg.L-1即可将U251细胞存活率显著降低至82.88%±1.24%(P<0.01),IC50为203.81 mg.L-1。(2)Pae对U251细胞增殖的抑制作用随时间延长而增强。(3)Pae与VP-16联合使用,可显著增强VP-16对U251的细胞毒性,当联用Pae剂量为15.63 mg.L-1时,VP-16的IC50值降低至376.81μmol.L-1,与单独使用时的IC50值(767.34μmol.L-1)相比降低幅度达50.89%。(4)Pae增强VP-16对U251细胞毒性的作用具有时间依赖性,随给药时间延长而逐渐增强。结论:Pae可抑制人胶质母细胞瘤U251细胞增殖。Pae与化疗药物VP-16联用时可以显著增强此化疗药物对胶质母细胞瘤的细胞毒性,提高胶质母细胞瘤细胞对化疗药物的敏感性。
Objective: To study the effect of paeonol (Pae) on the proliferation of human glioblastoma cells and its effect on chemosensitivity. Methods: The effects of Pae on the proliferation of human glioblastoma U251 cells in vitro were detected by water-soluble tetrazolium salt method. Pae and VP-16 were treated with different concentrations and time points to detect the effects of Pae on VP -16 cytotoxicity and calculate IC50 values using SPSS17.0 system software. Results: (1) Pae could inhibit the proliferation of human glioblastoma U251 cells in a dose-dependent manner. Pae concentration of 15.63 mg.L-1 significantly reduced the survival rate of U251 cells to 82.88% ± 1.24% (P <0.01), IC50 was 203.81 mg.L-1. (2) The inhibitory effect of Pae on U251 cell proliferation increased with time. (3) The combination of Pae and VP-16 significantly enhanced the cytotoxicity of VP-16 to U251. The IC50 of VP-16 decreased to 376.81μmol.L-1 when the Pae dose was 15.63 mg.L-1. 1, which was 50.89% lower than that of IC50 alone (767.34μmol.L-1). (4) Pae enhances the cytotoxicity of VP-16 to U251 cells in a time-dependent manner, and gradually increases with the administration time. Conclusion: Pae can inhibit the proliferation of human glioblastoma U251 cells. Pae and chemotherapy drug VP-16 can significantly enhance the chemotherapeutic drug on glioblastoma cytotoxicity and improve the sensitivity of glioblastoma cells to chemotherapy drugs.