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检测支原体污染是细胞培养中有待解决的一个问题。作者应用常见几种污染细胞培养的支原体(口腔支原体、精氨酸支原体、猪鼻支原体,莱氏无胆甾原体及唾液支原体,几种支原体占污染细胞培养支原体的96％)。为抗原,免疫了雌性BALB/c小鼠。将小鼠脾细胞与骨髓瘤细胞Xg3Ag 865融合成杂交瘤细胞,建立了30种抗支原体单克隆抗体杂交瘤细胞等。应用ELISA,
Detection of mycoplasma contamination is a problem to be solved in cell culture. The authors used several common mycoplasmas (Mycoplasma hyopneumoniae, Mycoplasma arginine, Mycoplasma hyopneumoniae, Choleraessela leucogenes and Salivary mycoplasma, which account for 96% of mycoplasma contaminated cell cultures) cultured in several common cell contaminated cells. As antigen, female BALB / c mice were immunized. The mouse spleen cells and myeloma cells Xg3Ag 865 fusion into hybridoma cells, established 30 kinds of anti-mycoplasma monoclonal antibody hybridoma cells. Apply ELISA,