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目的探讨转录共激活因子(TAZ)在结直肠癌患者外周血与肿瘤组织中表达,分析其关系及临床意义。方法选取经病理确诊为结直肠癌并行根治性手术患者85例为手术组,手术后确诊复发的患者35例作为复发组,同时选取年龄性别相匹配的健康人50例为对照组。取结直肠癌患者肿瘤组织及正常黏膜组织石蜡标本,采用免疫组织化学染色检测TAZ表达,酶联免疫吸附试验(ELISA)检测各组外周血中TAZ的水平。结果手术组患者术前外周血中TAZ水平明显高于对照组(P<0.05),手术后TAZ水平较对照组无明显变化(P>0.05),复发组患者外周血TAZ水平明显高于手术组术前(P<0.05)。手术组患者肿瘤组织中TAZ表达明显高于正常黏膜组织(P<0.05),复发组患者肿瘤组织中TAZ表达高于手术组(P<0.05)。手术组和复发组患者肿瘤组织与外周血TAZ水平呈正相关性(P<0.05)。结论监测TAZ水平对及时了解肿瘤复发有明显的参考价值。
Objective To investigate the expression of transcriptional coactivator (TAZ) in peripheral blood and tumor tissues of patients with colorectal cancer and to analyze its relationship and clinical significance. Methods Totally 85 patients with pathologically confirmed colorectal cancer undergoing radical operation were selected as surgical group and 35 patients with confirmed recurrence after operation as recurrence group. Meanwhile, 50 healthy and age-matched healthy individuals were selected as control group. Tumor specimens from patients with colorectal cancer and normal mucosa tissues were collected for immunohistochemical staining for TAZ expression. The levels of TAZ in peripheral blood were detected by enzyme linked immunosorbent assay (ELISA). Results The TAZ level in the peripheral blood of patients in the operation group was significantly higher than that in the control group (P <0.05), but the level of TAZ in the recurrence group was significantly higher than that of the control group (P> 0.05) Preoperative (P <0.05). The TAZ expression in the tumor tissue of the operation group was significantly higher than that of the normal mucosa (P <0.05). The TAZ expression in the tumor tissue of the recurrence group was higher than that in the operation group (P <0.05). There was a positive correlation between the tumor tissue and peripheral blood TAZ in surgical group and recurrent group (P <0.05). Conclusion Monitoring the level of TAZ has obvious reference value for timely understanding of tumor recurrence.