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粘附基质细胞层是骨髓长期培养(LTBMC)中原始祖细胞生存和增殖必不可少的。其中,干细胞因子(SCF)起了重要作用。为了探讨SCF在LTBMC中的作用,作者对小鼠骨髓长期培养作了研究。 方法:将培养液分为二种:一种为正常骨髓培养液(含有粘附基质层),一种为经照射的骨髓培养液(无粘附基质层),试验组中加入重组的小鼠造血干细胞因子(rrSCF),并设立对照组。根据培养两周后
Adhesion to the stromal cell layer is essential for the survival and proliferation of primitive progenitor cells in bone marrow long-term culture (LTBMC). Among them, stem cell factor (SCF) plays an important role. In order to investigate the role of SCF in LTBMC, we studied the long-term culture of mouse bone marrow. Methods: The culture medium was divided into two kinds: one kind of normal bone marrow culture solution (containing adherent matrix layer), one kind of irradiated bone marrow culture solution (no adhesion matrix layer), the experimental group was added recombinant mouse Hematopoietic stem cell factor (rrSCF), and set up a control group. After two weeks of training