草甘膦抗性真菌的分离与鉴定

• 作 者: 吴丹丹 张纯 冯莉 张妤 郭爱玲 田兴山
• 机 构: 广东省农业科学院植物保护研究所/广东省植物保护新技术重点实验室,华中农业大学食品科技学院,
• 出 处: 环境昆虫学报
• 发表日期: 2013年03期
• 关键词: 草甘膦 草甘膦抗性 淡紫拟青霉 抗性 多变根毛霉 rDNA-ITS序列 Paecilomyces 根毛霉 真菌菌株 发育学

采用含浓度梯度草甘膦选择性培养基平板分离法,从华南地区农田土壤中分离筛选出高抗草甘膦真菌菌株,通过传统的表型特征和系统发育学ITS序列分析对菌株进行鉴定。结果显示所筛选出的3株菌株分别为淡紫拟青霉Paecilomyces lilacinus,多变根毛霉Rhizomucor variabilis和黄曲霉Aspergillus flavus,抗性实验结果表明,它们对草甘膦具有良好的耐受性,分别为200mmol/L、400 mmol/L和500 mmol/L,可作为进一步获得抗草甘膦基因的菌株材料。

The glyphosate-resistant fungal strains were isolated from farmland soils in South China by plate culture method using concentration gradient glyphosate selective medium. The strains were identified by traditional phenotypic characteristics and phylogenetic ITS sequence analysis. The results showed that the three selected strains were Paecilomyces lilacinus, Rhizomucor variabilis and Aspergillus flavus, respectively. The results of their resistance test showed that they were well tolerant to glyphosate, respectively 200 mmol / L, 400 mmol / L and 500 mmol / L, which could be used as the material for further glyphosate resistant gene.