论文部分内容阅读
目的:研究活血通络汤预防性治疗激素性股骨头缺血性坏死(SANFH)兔造模过程中对碱性磷酸酶(AKP)、Jagged1表达的影响。方法:将192只日本大耳兔随机分为空白组、模型组、治疗组、预防组4组,各48只。除空白组外,均在第3周开始运用贺西京造模法造模,造模周期为6周。观察造模前、造模中、造模后相对应的时间点即第2、5、8周末家兔股骨头大体形态、血清AKP、Jagged1的动态变化。同时做股骨头镜检(光镜)、计算空骨陷窝率。结果:Jagged1表达方面,4组均呈逐渐递增趋势,模型组最高,预防组、治疗组两组间无可比性。AKP浓度方面,预防组、治疗组、模型组均呈递增趋势,预防组的AKP含量低于治疗组、模型组,模型组浓度最高。空骨陷窝率方面,预防组有下降趋势,治疗组、模型组均逐渐上升,但治疗组上升幅度小于模型组。结论:活血通络汤可以明显下调Jagged1、AKP的表达,促进新生血管的形成、促进骨钙量上升、促进骨代谢、减少骨钙流失、促进骨质成熟,有效改善股骨头局部缺血缺氧环境,从而有效干预股骨头缺血坏死的形成。
Objective: To study the effect of Huoxuetongluo Decoction on prophylactic treatment of alkaline phosphatase (AKP) and Jagged1 expression in rabbits with steroid-induced avascular necrosis of the femoral head (SANFH). Methods: A total of 192 Japanese large-eared rabbits were randomly divided into blank group, model group, treatment group and prevention group, with 48 rats in each group. In addition to the blank group, all began to use the third week of He Xijing modeling modeling, modeling cycle of 6 weeks. The morphological changes of rabbits’ femoral head, the serum AKP and Jagged1 at the end of the 2nd, 5th, and 8th weeks were observed before and after the model making. At the same time to do the femoral head microscopy (light microscope), calculate the empty bone lacuna. Results: In the Jagged1 expression group, the four groups showed a gradual increasing trend, the highest in the model group, no comparison between the two groups in the prevention group and the treatment group. AKP concentration, prevention group, treatment group, model group showed an increasing trend, the prevention group AKP content was lower than the treatment group, the model group, the model group highest concentration. In terms of empty lacunae, the prophylaxis group showed a downward trend, and both the treatment group and the model group gradually increased, but the increase rate in the treatment group was smaller than that in the model group. Conclusion: Huoxue Tongluo Decoction can obviously down-regulate the expression of Jagged1 and AKP, promote the formation of new blood vessel, promote the increase of bone calcium, promote the metabolism of bone, reduce the loss of bone calcium, promote bone maturation and effectively improve the ischemia and hypoxia of femoral head Environment, and thus effectively interfere with the formation of avascular necrosis.