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将高山森林土壤装入PVC管中(土壤有机层在上、矿质土壤层在下)培养10周,以高山森林土壤年均温为对照,采用室内人工气候箱分别模拟增温2和4℃,研究土壤微生物群落和土壤酶活性对温度升高的响应.结果表明:温度升高显著降低了土壤有机层中细菌、矿质土壤层中革兰氏阴性菌(G~-)PLFAs含量,但对土壤真菌无显著影响.温度升高引起革兰氏阳性菌和阴性菌比值(G~+/G~-)升高,改变了微生物群落结构.增温对漆酶、β-葡萄糖酶、酸性磷酸酶和N-乙酰葡糖胺糖苷酶活性没有显著影响.土壤微生物群落之间呈现出协同增长的趋势,真菌、细菌、G~+、G~-等微生物群落之间均呈显著正相关.土壤有机层中β-葡萄糖苷酶与土壤微生物群落对碳源利用的竞争,导致β-葡萄糖苷酶活性与土壤有机层细菌、真菌、G~+呈显著负相关.高山森林不同土壤微生物类群对增温的响应不同,细菌比真菌对温度的响应更敏感,真菌对增温有一定的耐受能力.
The alpine forest soil was filled into PVC pipe (organic layer on top and mineral soil layer on the bottom) for 10 weeks. The average annual soil temperature in alpine forest was used as control. The indoor artificial climate box was used to simulate the warming of 2 and 4 ℃ respectively. Soil microbial community and soil enzyme activity under elevated temperature.The results showed that PLFAs content of Gram-negative bacteria (G ~ -) in soil and organic matter layers decreased significantly with the increase of temperature, (G ~ + / G ~ -) increased, which changed the microbial community structure.When the temperature was increased, the activity of laccase, β-glucanase, acid phosphatase and N-acetylglucosaminidase activity had no significant effect on the soil microbial community showed a trend of synergic growth between fungi, bacteria, G ~ +, G ~ - and other microbial communities were significantly positive correlation between soil organic layer The competition of β-glucosidase and soil microbial community for utilization of carbon sources led to a significant negative correlation between β-glucosidase activity and bacteria, fungi and G ~ + in soil organic layer. The response is different than bacteria More sensitive response to the temperature, warming fungi on a certain tolerance.