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目的探讨C225单用及与顺铂(DDP)联用对于人子宫颈癌HeLa细胞裸鼠移植瘤生长的抑制作用及机制。方法将40只子宫颈癌HeLa细胞株裸鼠移植瘤模型随机分为实验对照组、C225组、DDP组和C225+DDP组,每组10只。各组裸鼠分别经腹腔注射生理盐水、C225(1mg/只·次)、DDP(5mg/kg·次)、和C225+DDP(C2251mg/只·次,DDP5mg/kg·次)每周2次,共4周。治疗期间定期测量肿瘤大小,观察裸鼠全身状况,实验结束时,将移植瘤完整取出,称取瘤重,计算抑瘤率,并用免疫组织化学技术检测各组肿瘤组织中PCNA、VEGF的表达。结果C225组、DDP组、C225+DDP组肿瘤的平均体积均显著小于实验对照组(P=0.008,P=0.001,P=0.000);C225+DDP组的肿瘤体积亦显著小于DDP组与C225组(P=0.025,P=0.014);各组平均瘤重分别为2.67±0.18g、1.84±0.37g、1.57±0.18g、1.2±0.09g,C225组、DDP组、C225+DDP组与实验对照组之间具有显著性差异(P=0.015,P=0.001,P=0.000),抑瘤率分别为31.09%、41.2%、55.06%。免疫组化结果显示,PCNA阳性表达率在C225组、DDP组与C225+DDP组较实验对照组显著降低(P=0.033,P=0.011,P=0.003);VEGF的表达率经C225与DDP单用及联合治疗后较实验对照组显著降低(P=0.033,P=0.033,P=0.003)。结论C225与DDP均可抑制人子宫颈癌HeLa细胞株裸鼠移植瘤生长,两药联合应用的抑瘤效果优于两药单用;C225单用及与DDP联用后可显著抑制PCNA与VEGF的表达水平。
Objective To investigate the inhibitory effect of C225 alone or in combination with cisplatin (DDP) on the growth of human cervical carcinoma HeLa cell xenografts in nude mice. Methods Forty cervical cancer HeLa cells were randomly divided into experimental control group, C225 group, DDP group and C225 + DDP group, with 10 mice in each group. The nude mice in each group were injected intraperitoneally with normal saline (C225 (1mg / dose), DDP (5mg / kg), and C225 + DDP (C2251mg / , A total of 4 weeks. At the end of the experiment, the transplanted tumor was completely removed, the tumor weight was weighed, the tumor inhibition rate was calculated, and the expression of PCNA and VEGF in each group was detected by immunohistochemistry. Results The average volume of tumor in C225, DDP and C225 + DDP groups was significantly smaller than that in experimental group (P = 0.008, P = 0.001, P = 0.000) (P = 0.025, P = 0.014). The average tumor weight in each group was 2.67 ± 0.18g, 1.84 ± 0.37g, 1.57 ± 0.18g and 1.2 ± 0.09g respectively. Compared with control group, C225 group, DDP group and C225 + DDP group There was significant difference between the two groups (P = 0.015, P = 0.001, P = 0.000). The tumor inhibition rates were 31.09%, 41.2% and 55.06%, respectively. Immunohistochemistry showed that the positive expression rate of PCNA in C225 group, DDP group and C225 + DDP group was significantly lower than the experimental control group (P = 0.033, P = 0.011, P = 0.003) Compared with the experimental group, the combined treatment decreased significantly (P = 0.033, P = 0.033, P = 0.003). Conclusions Both C225 and DDP can inhibit the growth of human cervical cancer HeLa cell xenografts in nude mice, and the antitumor effect of the two drugs combined is better than the two drugs alone; C225 alone and in combination with DDP can significantly inhibit the growth of PCNA and VEGF The level of expression.