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目的分析抗ABCA1自身抗体在SLE患者动脉粥样硬化形成中的作用。方法选取郑州大学第一附属医院风湿科2014年1月至2015年6月收治的80例SLE患者,通过细胞培养和免疫印迹法检测ABCA1抗体阳性情况,通过蛋白A凝胶层析柱纯化Ig G抗体检测胆固醇流出率。结果 SLE患者ABCA1抗体阳性率明显高于体检健康者,差异有统计学意义(P<0.05)。SLE伴动脉粥样硬化组ABCA1抗体阳性率高于SLE不伴动脉粥样硬化组,差异有统计学意义(P<0.05)。抗ABCA1抗体阳性Ig G干预组胆固醇流出率低于体检健康者Ig G干预组,差异有统计学意义(P<0.05)。结论抗ABCA1抗体对SLE患者胞内胆固醇的流出具有抑制作用,并可能由此促进动脉粥样硬化的形成。
Objective To analyze the role of anti-ABCA1 autoantibodies in the development of atherosclerosis in SLE patients. Methods Eighty SLE patients admitted to Department of Rheumatology, the First Affiliated Hospital of Zhengzhou University from January 2014 to June 2015 were enrolled in this study. The positive expression of ABCA1 antibody was detected by cell culture and immunoblotting, and IgA was purified by protein A gel chromatography Antibody detection of cholesterol efflux rate. Results The positive rate of ABCA1 antibody in SLE patients was significantly higher than that in healthy subjects (P <0.05). The positive rate of ABCA1 antibody in SLE with atherosclerosis group was higher than that in SLE without atherosclerosis group, the difference was statistically significant (P <0.05). The cholesterol efflux rate in the anti-ABCA1 antibody-positive Ig G intervention group was lower than that of the Ig G intervention group, the difference was statistically significant (P <0.05). Conclusion Anti-ABCA1 antibody can inhibit the intracellular cholesterol efflux in patients with SLE and may thus promote the formation of atherosclerosis.