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目的：选用慢粒患者的原代血细胞，经细胞因子组合联合诱导，生成携带白血病抗原信息的树突状细胞（Ｄｅｎｄｒｉ－ｔｉｃ Ｃｅｌｌ ， ＤＣ），为ＤＣ疗法在临床上的应用提供理论依据。方法：慢粒（急变期）病人的ＰＢＭＣ用ＧＭ－ＣＳＦ＋ＩＬ－４＋ＴＮＦα诱导后进行形态和细胞表型的鉴定。应用同种混合淋巴细胞反应和细胞毒性实验，观察诱生之ＤＣ刺激淋巴细胞增殖的能力以及介导抗白血病特异性细胞毒性反应的能力。利用酶联免疫吸附实验检测ＤＣ产生ＩＬ－１２及协助产生ＩＦＮ－γ的能力。结果： 诱生ＤＣ均具有ＤＣ的形态特征和超微结构。表达ＤＣ相关表面分化抗原。并可刺激Ｔ淋巴细胞产生较强的增殖效应。诱导出针对自身肿瘤靶细胞的特异性ＣＴＬ反应。同时可不同程度的分泌ＩＬ－１２和可协助Ｔ细胞产生ＩＦＮ－γ。结论： 慢粒细胞在适当的细胞因子的诱导下，可分化为具有ＤＣ形态的细胞，后者可诱导出高效而特异的抗肿瘤免疫。
OBJECTIVE: To select dendritic cells (DCs) carrying leukemia antigen information by the combination of cytokines and induce the primary blood cells of patients with chronic myeloid leukemia (DC) to provide a theoretical basis for the clinical application of DC therapy. Methods: The morphological and cell phenotypes of PBMCs from CML patients induced by GM-CSF + IL-4 + TNFα were determined. The same kind of mixed lymphocyte reaction and cytotoxicity test were used to observe the ability of induced DCs to stimulate lymphocyte proliferation and the ability of mediating anti-leukemia-specific cytotoxic reaction. Enzyme-linked immunosorbent assay was used to detect the ability of DCs to produce IL-12 and to help produce IFN-γ. Results: Induced DC possessed the morphological features and ultrastructure of DC. Expression of DC-associated surface differentiation antigen. And can stimulate T lymphocytes produce a strong proliferative effect. Induced a specific CTL response to its own tumor target cells. At the same time, different levels of IL-12 can be secreted and T-cells can help produce IFN-γ. CONCLUSION: CML can differentiate into cells with DC morphology under the induction of appropriate cytokines, which can induce efficient and specific anti-tumor immunity.