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[目的]确定荞麦种子发芽的适宜甲基磺酸乙酯(EMS)诱变剂量,为进一步获得荞麦优良性状并培育符合EMS诱变育种需求的新品种打下坚实的基础。[方法]以山西省当地的苦荞[Fagopyrum tataricum(L.)Gaertn]品种晋荞麦4号种子为材料,用不同浓度梯度(0.3%、0.5%、0.7%、1.0%、1.5%、1.7%)EMS诱变处理不同时间(4、8、12 h),分析苦荞种子发芽率和发芽势。[结果]随着EMS诱变剂浓度的增加,晋荞麦4号种子发芽势、相对发芽势和发芽率、相对发芽率均呈下降趋势,当EMS浓度为0.3%~1.0%时与对照没有显著差异,当EMS浓度为1.5%和1.7%时与对照差异极显著EMS处理48 h对晋荞麦4号种子发芽率、相对发芽率都没有大的影响,当处理时间为12 h时,与对照有显著差异;对于发芽势和相对发芽势,这3个处理时间没有显著差异。[结论]EMS诱变苦荞的半致死的适宜浓度为1.7%,适宜的处理时间为12 h。
[Objective] The study aimed to determine the suitable EMS mutagenesis dose for buckwheat seeds, which laid a solid foundation for further obtaining the excellent traits of buckwheat and cultivating new varieties that meet EMS mutagenesis needs. [Method] The seeds of Fagopyrum tataricum (L.) Gaertn from Shanxi Province were used as the materials, and the seedlings of the seeds were treated with different concentration gradients (0.3%, 0.5%, 0.7%, 1.0%, 1.5%, 1.7% ) EMS mutagenesis treatment at different times (4,8,12 h), analysis of germination rate and germination potential of tartary buckwheat seeds. [Result] With the increase of EMS mutagen concentration, the germination potential, relative germination potential, germination rate and relative germination rate of Jin buckwheat seed No.4 showed a decreasing trend. When the EMS concentration was 0.3% ~ 1.0%, there was no significant difference with the control The difference was significant when EMS concentration was 1.5% and 1.7%, respectively. EMS treatment for 48 h had no significant effect on the germination rate and relative germination rate of Jin buckwheat 4 seeds. When the treatment time was 12 h, Significant differences; for the germination potential and relative germination potential, these three treatment time is not significantly different. [Conclusion] The suitable semi-lethal concentration of EMS for mutagenesis was 1.7% and the suitable treatment time was 12 h.