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目的探讨应用PCR-SSCP分析我国不同地区常见嗜尸蝇类,揭示其亲源关系及基因差异。方法采集了中国部分城市,即广州、深圳、阳江、南京、长春、宜昌、北京、武汉、成都等地的常见嗜尸蝇类:家蝇(Musc domestica),丝光绿蝇(Lucilia sericata),大头金蝇(Chrysomya megacephala),黑尾麻蝇(Helicophagella melanura),棕尾麻蝇(Boettcherisca peregrina)等。用PCR-SSCP分析我国不同嗜尸蝇类的rDNA-ITS2基因片段。结果PCR-SSCP分析结果显示,Chrysomya megacephata,Aldrichina grahami(巨尾阿丽蝇),Helicophagella melanura,Musc domestica,Boettcherisca peregrina rDNA-ITS2基因的单链DNA(ssDNA)迁移率均有明显差异,我国不同地区常见嗜尸蝇种类rDNA-ITS2基因单链DNA迁移图型呈现多态性。结论我国不同地区常见嗜尸蝇种类的rDNA-ITS2基因存在种内、种间及地理的基因多态性。
Objective To explore the use of PCR-SSCP analysis of common flies in different parts of China to reveal their relationship and genetic differences between parents. Methods The common pestilents of Musc domestica, Lucilia sericata, and some other common flies were collected from some cities in China, namely Guangzhou, Shenzhen, Yangjiang, Nanjing, Changchun, Yichang, Beijing, Wuhan and Chengdu. Chrysomya megacephala, Helicophagella melanura, Boettcherisca peregrina and the like. The rDNA-ITS2 gene fragments of different zombie flies in China were analyzed by PCR-SSCP. Results The results of PCR-SSCP showed that there were significant differences in the single-stranded DNA (ssDNA) mobility of Chrysomya megacephata, Aldrichina grahami, Helicophagella melanura, Musc domestica and Boettcherisca peregrina rDNA-ITS2 genes. The common type of rhesus fly species rDNA-ITS2 single-stranded DNA migration patterns showed polymorphism. Conclusion There are intraspecific, interspecific and geographical genetic polymorphisms in the rDNA-ITS2 gene of the common archezoocarp species in different regions of China.