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创制抗黄曲霉种质并建立相关分子标记辅助选择技术是深化抗性遗传改良的基础。本研究以源于对黄曲霉具有不同抗性的花生品种中花10号(感病种质)与ICG12625(抗性种质)杂交构建的重组自交系(RIL)群体为材料,通过重复接种鉴定,发现所涉及重组自交系群体的黄曲霉抗性存在很大分化,遗传分析结果显示,侵染和产毒抗性均符合2对主基因+多基因遗传模型;获得侵染指数较低的RIL家系1个(QT401),毒素浓度较低的家系4个(QT344、QT389、QT477和QT483)。通过对RIL群体的多态性检测并应用Joinmap3.0软件,构建了1张由458个SSR标记位点组成、包含20个连锁群、总长为1 165.45c M的遗传连锁图。结合抗性鉴定数据,应用Win QTLCart2.0软件的复合区间作图(CIM)法,获得了6个与黄曲霉侵染抗性相关的QTL和10个与产毒抗性相关的QTL,贡献率在6.34%~12.00%之间。
Establishing anti-aflatoxin germplasm and establishing related molecular marker-assisted selection techniques are the basis for deepening the genetic improvement of resistance. In this study, a recombinant inbred line (RIL) population derived from hybrids of Zhonghua10 (susceptible germplasm) and ICG12625 (resistant germplasm) derived from peanut with different resistance to Aspergillus flavus was used as materials. Identification and found that the recombinant inbred line population involved in the resistance of Aspergillus flavonoids there is a great differentiation, genetic analysis showed that infection and toxin resistance are in line with the two major genes + polygene genetic model; access to infection index is low 1 QTL (QT401) and 4 low QTLs (QT344, QT389, QT477 and QT483). Based on the polymorphism detection of RIL population and using Joinmap3.0 software, a genetic linkage map consisting of 458 SSR marker loci containing 20 linkage groups and a total length of 1 165.45c M was constructed. Combined with the resistance identification data, six QTLs related to infection resistance of Aspergillus flavus and 10 QTLs related to toxigenicity were obtained by the composite interval mapping (CIM) method of Win QTLCart2.0 software. The contribution rate Between 6.34% ~ 12.00%.