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Objective:To develop allenualed slrains of Salmonella enterica serorar Typhi(S.typhi) for the candidate vaccine by osmolar stress.Mothods:S.typhi SS3 and SS5 strains were isolated from asymptomatic typhoid carriers in Mamakkal,Tamil Nadu.India.Both strains were grown in LB(Luria Bertani) medium supplemented with various concentration of NaCl(0.1- 0.7M) respectively.The effecl of osmolar stress was determined at molecular level by PCR using MCR 06 and MCR07 primers corresponding to ompR with chromosomal DNA of S.typhi SS3 and SS5 strains.Attenuation by osmolar stress results in deletion mutation of the.S.typhi slrains was determined by agglutination assays,precipitation method.SDS PAGE analysis and by animal models.Results:The 799 bp amplified ompR gene product from wild type S.typhi SS3 and SS5 illustrate the presence of virulent gene.Interestingly,there was only a 282 bp amplified product from S.typhi SS3 and SS5 grown in the presence of 0.5.0.6 and 0.7 M NaCl.This illustrates the occurrence of deletion mutation in ompR gene al high concentration of NaCl.Furthermore,both the wildtype and mutant S.typhi outer membrane SDS-PAGF.profile reveals the differences in the expression of ompF.ompC and ompA proteins.In mice,wild type and mutant strains lethal dose(LD_(50)) were determined.The mice died within 72 h when both the wild type strains were injected intraperitoneally with 3 log CFU-mL~(-1).When the mice were injected with the mutants in same dosage,no clinical symptoms were observed;whereas the serum antibodv litre was elicited within two weeks indicated that the mutants have the ability to induce protective humoral immune response.These results suggest that S.typhi SS3 and SS5 may bo used as good candidate strains for the development of live attenuated vaccine against salmonellosis.Conclusions:This study demonstrates that the S.typhi strains were allenualed and could be good vaccine candidates in future.
Objective: To develop allenualed slrains of Salmonella enterica serorar Typhi (S. typhi) for the candidate vaccine by osmolar stress. Methods: S.typhi SS3 and SS5 strains were isolated from asymptomatic typhoid carriers in Mamakkal, Tamil Nadu. India.Both strains were grown in LB (Luria Bertani) medium supplemented with various concentrations of NaCl (0.1-0.7 M) respectively.The effector of osmolar stress was determined at molecular level by PCR using MCR 06 and MCR07 primers corresponding to ompR with chromosomal DNA of S.typhi SS3 and SS5, A. Attenuation by osmolar stress results in deletion mutation of the S. typhi slrains was determined by agglutination assays, precipitation method. SDS PAGE analysis and by animal models. Results: The 799 bpmplified ompR gene product from wild type S .typhi SS3 and SS5 illustrate the presence of virulent gene.Interestingly, there was only a 282 bp amplified product from S.typhi SS3 and SS5 grown in the presence of 0.5.0.6 and 0.7 M NaCl. This illustrates the occ urrence of deletion mutation in ompR gene al high concentration of NaCl.Furthermore, both the wildtype and mutant S. typhi outer membrane SDS-PAGF.profile reveals the differences in the expression of ompF.ompC and ompA proteins. in mice, wild type and mutant mice lethal dose (LD_ (50)) were determined. The mice were within 72 h when both the wild type strains were injected intraperitoneally with 3 log CFU-mL ~ (-1) .When the mice were injected with the mutants in same dosage, no clinical symptoms were observed; whereas the serum antibodv litre was elicited within two weeks indicated that the mutants have the ability to induce protective humoral immune response .sese results suggest that S.typhi SS3 and SS5 may bo used as good candidate strains for the development of live attenuated vaccine against salmonellosis. Conclusions: This study demonstrates that the S. typhi strains were allenualed and could be a good vaccine candidates in future.