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目的:探讨荣肝合剂对刀豆蛋白A(Con A)诱导的急性免疫性肝损伤小鼠病理组织学及T淋巴细胞亚群的影响。方法:乙型肝炎病毒(HBV)转基因小鼠60只,随机分为6组(模型组、正常组、荣肝合剂组、茵陈蒿汤组、茵陈组、联苯双酯组),每组10只,造模前14天,模型组、正常组小鼠给予生理盐水灌胃,其余组分别给予荣肝合剂、茵陈蒿汤、单味茵陈煎液、联苯双酯溶液,每日灌胃给药干预。末次灌胃给药后1h,正常组小鼠给予磷酸盐缓冲液(PBS)尾静脉注射,其余各组按照Con A3μg/g体重尾静脉注射造模。造模给药后8h处死动物取血或组织标本检测丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)、总胆红素(TBil)、组织病理学(光镜)及T淋巴细胞亚群等指标。结果:模型组各指标与正常组比较,差异均有统计学意义(P<0.05,P<0.01);与模型组比较,荣肝合剂组、联苯双酯组小鼠ALT、AST、TBil水平显著降低(P<0.01),荣肝合剂组、茵陈蒿汤小鼠组肝细胞变性、坏死及炎细胞浸润程度较轻;各药物干预组CD4~+CD8~-T淋巴细胞所占比例均有升高,以荣肝合剂最显著,有显著性差异(P<0.05);荣肝合剂组CD4~+CD8~+T淋巴细胞所占比例下降(P<0.05);各中药组均可升高CD4~+/CD8~+T细胞比例(P<0.05)。同时,荣肝合剂CD4~+CD8~-T淋巴细胞所占比例于其他药物干预组比较差异有显著性意义(P<0.05),荣肝合剂组CD4~+CD8~+T细胞所占比例与联苯双酯组比较有显著性差异(P<0.05),荣肝合剂组CD4~+/CD8~+T细胞比值与茵陈蒿汤组比较差异有显著性意义(P<0.05)。结论:荣肝合剂对刀豆蛋白A所致转基因小鼠急性免疫性肝损伤具有降酶作用,同时对于肝组织有保护作用,可以减轻肝组织的炎症与坏死,其机理与调节T淋巴细胞亚群功能相关。
Objective: To investigate the effect of Ronggan mixture on histopathology and T lymphocyte subsets of Con A-induced acute immunological liver injury in mice. Methods: Sixty transgenic mice with hepatitis B virus (HBV) were randomly divided into 6 groups (model group, normal group, Ronggan Mixture group, Yinchenhao Decoction group, Yinchen group and Bifendate group) Group 10, 14 days before modeling, the model group, the normal group of mice given saline gavage, and the remaining group were given Ronggan mixture, Yinchenhao decoction, single Yincao decoction, bifendate solution, each Daily gavage intervention. One hour after the last intragastric administration, the mice in the normal group were given phosphate buffered saline (PBS) injected into the tail vein and the rest were injected into the caudal vein with Con A3 μg / g body weight. The animals were sacrificed at 8h after model administration to determine the levels of ALT, AST, TBil, histopathology (light microscopy) And T lymphocyte subsets and other indicators. Results: Compared with the normal group, the indexes in the model group were significantly different (P <0.05, P <0.01). Compared with the model group, the ALT, AST, TBil levels (P <0.01). The hepatocellular degeneration, necrosis and infiltration of inflammatory cells in Ronggan Mixture group and Yinchenhao decoction group were lighter. The proportion of CD4 ~ + CD8 ~ -T lymphocytes in each drug intervention group was (P <0.05). The proportion of CD4 ~ + CD8 ~ + T lymphocytes in Ronggan Mixture group decreased significantly (P <0.05), while that in each TCM group increased High CD4 ~ + / CD8 ~ + T cell ratio (P <0.05). At the same time, the percentage of CD4 ~ + CD8 ~ -T lymphocytes of Ronggan Mixture was significantly different from other drug intervention groups (P <0.05). The proportion of CD4 ~ + CD8 ~ + T cells in Ronggan Mixture group was significantly correlated with (P <0.05). The ratio of CD4 ~ + / CD8 ~ + T cells in Ronggan Mixture group was significantly different from that in Yinchenhao Decoction group (P <0.05). CONCLUSION: Ronggan Mixture has a reducing enzyme effect on acute immunological liver injury induced by concanavalin A. It also has a protective effect on liver tissue and can reduce the inflammation and necrosis of liver tissue. The mechanism and regulation of T lymphocyte subsets Group function related.