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在提取我国１２个省主产棉区２６个菌系ｇＤＮＡ的基础上，采用ＯＰＹ和ＯＰＢ两组随机引物，对这些菌系的基因组进行了ＰＣＲ扩增和聚类分析，４０个引物中，１２个引物扩增出满意的适合于分析的多态性谱带。聚类分析将２６个菌系分为Ａ、Ｂ两个群，Ｖａ菌系（Ｖｅｒｔｉｃｉｌｉｕｍａｌｂｏａｔｒｕｍ）属于单独的Ａ群，Ｂ群则包括了所有的大丽轮枝菌系（Ｖｅｒｔｉｃｉｌｉｕｍｄａｈｌｉａｅ）。Ｂ群又分为Ｂａ（Ｃｓ、Ｙｃ）及Ｂｂ两个亚群。Ｂｂ亚群下面分３个组，Ｂｂ１（ＡＶ２、ＡＶ３及Ｌｙ）、Ｂｂ２（Ｓａ、Ｊｉ、Ｋｅ及Ｙｃ）和Ｂｂ３。Ｂｂ３是最大的一个组，包括供试的３个大丽轮枝菌落叶型的３个菌系（ＶＤ－８、Ｔ９、Ｖ４４）和其他大部分非落叶型的菌系。不同的单孢之间ＲＡＰＤ分析的结果（引物ＯＰＹ０６检测出单孢之间２．０６ｋｂ的差异条带）从分子水平证实了单孢间变异的发生。对河南安阳４个菌株单独聚类分析则表现出致病性测定与ＲＡＰＤ分析有一定的相关性。
Based on the gDNA of 26 bacterial strains in the main cotton producing areas in 12 provinces of China, the genomes of these strains were amplified by PCR and cluster analysis using random primers OPY and OPB. Of the 40 primers, 12 A primer amplified a satisfactory polymorphism band suitable for analysis. Cluster analysis divided 26 bacterial strains into A and B groups, while Va strain belonged to A group alone, while B group included all Verticilium dahliae strains. Group B is divided into Ba (Cs, Yc) and Bb two subgroups. Bb1 (AV2, AV3 and Ly), Bb2 (Sa, Ji, Ke and Yc) and Bb3 were subdivided into three subgroups. Bb3 is the largest group, consisting of 3 strains (VD-8, T9, V44) of the three deciduous Rhizoctonia species tested and the other most non-deciduous ones. The results of RAPD analysis (different bands of 2.06 kb between single spores detected by primer OPY06) among different monospores confirmed the occurrence of intermolecular mutation at the molecular level. The clustering analysis of four isolates of Anyang in Henan showed that the pathogenicity test had some correlation with RAPD analysis.